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TitleA Ca-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes.
Journal, issue, pagesJ Biol Chem, Vol. 298, Issue 6, Page 101967, Year 2022
Publish dateApr 20, 2022
AuthorsKazutoshi Tani / Kazumi Kobayashi / Naoki Hosogi / Xuan-Cheng Ji / Sakiko Nagashima / Kenji V P Nagashima / Airi Izumida / Kazuhito Inoue / Yusuke Tsukatani / Ryo Kanno / Malgorzata Hall / Long-Jiang Yu / Isamu Ishikawa / Yoshihiro Okura / Michael T Madigan / Akira Mizoguchi / Bruno M Humbel / Yukihiro Kimura / Zheng-Yu Wang-Otomo /
PubMed AbstractThe mildly thermophilic purple phototrophic bacterium Allochromatium tepidum provides a unique model for investigating various intermediate phenotypes observed between those of thermophilic and ...The mildly thermophilic purple phototrophic bacterium Allochromatium tepidum provides a unique model for investigating various intermediate phenotypes observed between those of thermophilic and mesophilic counterparts. The core light-harvesting (LH1) complex from A. tepidum exhibits an absorption maximum at 890 nm and mildly enhanced thermostability, both of which are Ca-dependent. However, it is unknown what structural determinants might contribute to these properties. Here, we present a cryo-EM structure of the reaction center-associated LH1 complex at 2.81 Å resolution, in which we identify multiple pigment-binding α- and β-polypeptides within an LH1 ring. Of the 16 α-polypeptides, we show that six (α1) bind Ca along with β1- or β3-polypeptides to form the Ca-binding sites. This structure differs from that of fully Ca-bound LH1 from Thermochromatium tepidum, enabling determination of the minimum structural requirements for Ca-binding. We also identified three amino acids (Trp44, Asp47, and Ile49) in the C-terminal region of the A. tepidum α1-polypeptide that ligate each Ca ion, forming a Ca-binding WxxDxI motif that is conserved in all Ca-bound LH1 α-polypeptides from other species with reported structures. The partial Ca-bound structure further explains the unusual phenotypic properties observed for this bacterium in terms of its Ca-requirements for thermostability, spectroscopy, and phototrophic growth, and supports the hypothesis that A. tepidum may represent a "transitional" species between mesophilic and thermophilic purple sulfur bacteria. The characteristic arrangement of multiple αβ-polypeptides also suggests a mechanism of molecular recognition in the expression and/or assembly of the LH1 complex that could be regulated through interactions with reaction center subunits.
External linksJ Biol Chem / PubMed:35460693 / PubMed Central
MethodsEM (single particle)
Resolution2.81 Å
Structure data

32100

7vrj

EMDB-32100, PDB-7vrj:
STRUCTURE OF PHOTOSYNTHETIC LH1-RC SUPER-COMPLEX OF Allochromatium tepidum
Method: EM (single particle) / Resolution: 2.81 Å

Chemicals

ChemComp-HEC:
HEME C

ChemComp-MG:
Unknown entry

ChemComp-DGA:
DIACYL GLYCEROL

ChemComp-PLM:
PALMITIC ACID

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM

ChemComp-BCL:
BACTERIOCHLOROPHYLL A

ChemComp-BPH:
BACTERIOPHEOPHYTIN A

ChemComp-UQ8:
Ubiquinone-8

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-FE:
Unknown entry

ChemComp-MQ8:
MENAQUINONE 8

ChemComp-CRT:
SPIRILLOXANTHIN

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-CA:
Unknown entry

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM

ChemComp-HOH:
WATER

Source
  • allochromatium tepidum (bacteria)
KeywordsPHOTOSYNTHESIS / LH1-RC COMPLEX / PURPLE BACTERIA

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