[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleAssembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy.
Journal, issue, pagesElife, Vol. 11, Year 2022
Publish dateMar 4, 2022
AuthorsSofia Lövestam / Fujiet Adrian Koh / Bart van Knippenberg / Abhay Kotecha / Alexey G Murzin / Michel Goedert / Sjors H W Scheres /
PubMed AbstractAbundant filamentous inclusions of tau are characteristic of more than 20 neurodegenerative diseases that are collectively termed tauopathies. Electron cryo-microscopy (cryo-EM) structures of tau ...Abundant filamentous inclusions of tau are characteristic of more than 20 neurodegenerative diseases that are collectively termed tauopathies. Electron cryo-microscopy (cryo-EM) structures of tau amyloid filaments from human brain revealed that distinct tau folds characterise many different diseases. A lack of laboratory-based model systems to generate these structures has hampered efforts to uncover the molecular mechanisms that underlie tauopathies. Here, we report in vitro assembly conditions with recombinant tau that replicate the structures of filaments from both Alzheimer's disease (AD) and chronic traumatic encephalopathy (CTE), as determined by cryo-EM. Our results suggest that post-translational modifications of tau modulate filament assembly, and that previously observed additional densities in AD and CTE filaments may arise from the presence of inorganic salts, like phosphates and sodium chloride. In vitro assembly of tau into disease-relevant filaments will facilitate studies to determine their roles in different diseases, as well as the development of compounds that specifically bind to these structures or prevent their formation.
External linksElife / PubMed:35244536 / PubMed Central
MethodsEM (helical sym.)
Resolution1.86 - 3.4 Å
Structure data

EMDB-14023, PDB-7qjv:
In vitro assembled tau filaments into Quadruple Helical Filaments type 1 (2c)
Method: EM (helical sym.) / Resolution: 3.29 Å

EMDB-14024, PDB-7qjw:
In vitro assembled tau filaments with structures like chronic traumatic encephalopathy type II (8a)
Method: EM (helical sym.) / Resolution: 2.81 Å

EMDB-14025, PDB-7qjx:
In vitro assembled 297-394 tau filaments, 700 rpm (34b)
Method: EM (helical sym.) / Resolution: 2.99 Å

EMDB-14026, PDB-7qjy:
In vitro assembled 266/297 - 391 tau filaments with LiCl (9a)
Method: EM (helical sym.) / Resolution: 3.14 Å

EMDB-14027, PDB-7qjz:
In vitro assembled 266/297 - 391 tau filaments with LiCl (9b)
Method: EM (helical sym.) / Resolution: 3.4 Å

EMDB-14028, PDB-7qk1:
In vitro assembled 297-394 tau filaments in PBS (35d)
Method: EM (helical sym.) / Resolution: 3.03 Å

EMDB-14029, PDB-7qk2:
In vitro assembled 300-391 tau filaments in PBS (36a)
Method: EM (helical sym.) / Resolution: 2.61 Å

EMDB-14030, PDB-7qk3:
In vitro assembled 258-391 tau filaments, 700 rpm (38a)
Method: EM (helical sym.) / Resolution: 2.44 Å

EMDB-14038, PDB-7qk5:
In vitro assembled 266/297 - 391 tau filaments with KCl (10a)
Method: EM (helical sym.) / Resolution: 1.92 Å

EMDB-14039, PDB-7qk6:
In vitro assembled 258-391 tau filaments with heparan sulfate, 700 rpm (40a)
Method: EM (helical sym.) / Resolution: 2.27 Å

EMDB-14040, PDB-7qkf:
In vitro assembled 266/297 - 391 tau filaments with CuCl2 (12a)
Method: EM (helical sym.) / Resolution: 2.83 Å

EMDB-14041, PDB-7qkg:
In vitro assembled 258-391 tau filaments with phosphoglycerate, 700 rpm (39a)
Method: EM (helical sym.) / Resolution: 3.36 Å

EMDB-14042, PDB-7qkh:
In vitro assembled 258-391 tau filaments with sodium azide, (41a)
Method: EM (helical sym.) / Resolution: 3.17 Å

EMDB-14043, PDB-7qki:
In vitro assembled 297-408 S396D S400D T403D S404D tau filaments (42a)
Method: EM (helical sym.) / Resolution: 3.13 Å

EMDB-14044, PDB-7qkj:
In vitro assembled 266/297 - 391 tau filaments with MgCl2 and NaCl (14b)
Method: EM (helical sym.) / Resolution: 3.26 Å

EMDB-14045, PDB-7qkk:
In vitro assembled 258-391 tau filaments with phosphoglycerate, 700 rpm (39a)
Method: EM (helical sym.) / Resolution: 2.8 Å

EMDB-14046, PDB-7qkl:
In vitro assembled 266/297 - 391 tau filaments with ZnCl2 (11a)
Method: EM (helical sym.) / Resolution: 2.07 Å

EMDB-14047, PDB-7qkm:
In vitro assembled 266-391 S356D tau filaments with NaCl (45a)
Method: EM (helical sym.) / Resolution: 2.66 Å

EMDB-14053, PDB-7qku:
in vitro assembled 266/297 - 391 tau filaments with MgCl2 and NaCl (14a)
Method: EM (helical sym.) / Resolution: 2.57 Å

EMDB-14054, PDB-7qkv:
In vitro assembled tau filaments with MgSO4 and NaCl (15a)
Method: EM (helical sym.) / Resolution: 3.23 Å

EMDB-14055, PDB-7qkw:
In vitro assembled 266-391 S356D tau filaments with KCl (44a)
Method: EM (helical sym.) / Resolution: 2.32 Å

EMDB-14056, PDB-7qkx:
In vitro assembled 266/297 - 391 tau filaments with MgSO4 and NaCl (15b)
Method: EM (helical sym.) / Resolution: 3.16 Å

EMDB-14057, PDB-7qky:
In vitro assembled 0N4R tau filaments with phosphoserine (47a)
Method: EM (helical sym.) / Resolution: 1.86 Å

EMDB-14058, PDB-7qkz:
In vitro assembled 305-379 tau filaments (27a)
Method: EM (helical sym.) / Resolution: 2.65 Å

EMDB-14059, PDB-7ql0:
In vitro assembled 266/297 - 391 tau filaments with MgSO4 and NaCl (15c)
Method: EM (helical sym.) / Resolution: 3.13 Å

EMDB-14060, PDB-7ql1:
In vitro assembled 266-391 tau filaments in PBS (23a)
Method: EM (helical sym.) / Resolution: 3.34 Å

EMDB-14061, PDB-7ql2:
In vitro assembled 244-391 tau filaments with Na2P2O7 (20a)
Method: EM (helical sym.) / Resolution: 2.95 Å

EMDB-14062, PDB-7ql3:
in vitro assembled 266/297 - 391 tau filaments with NaCl (8b)
Method: EM (helical sym.) / Resolution: 3.32 Å

EMDB-14063, PDB-7ql4:
in vitro assembled tau filaments with structures like Paired Helical Filaments from Alzheimer's Disease
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-14316, PDB-7r4t:
In vitro assembled 266/297 - 391 tau filaments with NaHCO3 and NaCl (16a)
Method: EM (helical sym.) / Resolution: 2.75 Å

EMDB-14320, PDB-7r5h:
In vitro assembled 266/297 - 391 tau filaments with KCl (10b)
Method: EM (helical sym.) / Resolution: 2.59 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-K:
Unknown entry

Source
  • homo sapiens (human)
KeywordsPROTEIN FIBRIL / Neurodegeneration / Amyloid / Tau / Alzheimer's Disease / Chronic traumatic encephalopathy

+
About Yorodumi Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242):Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more