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Structure paper

TitleClamping of DNA shuts the condensin neck gate.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 119, Issue 14, Page e2120006119, Year 2022
Publish dateApr 5, 2022
AuthorsByung-Gil Lee / James Rhodes / Jan Löwe /
PubMed AbstractSignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex ...SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges loops in DNA through loop extrusion. Our work resolves the atomic structure of a DNA-bound state of condensin in which ATP has not been hydrolyzed. The DNA is clamped within a compartment that has been reported previously in other structural maintenance of chromosomes (SMC) complexes, including Rad50, cohesin, and MukBEF. With the caveat of important differences, it means that all SMC complexes cycle through at least some similar states and undergo similar conformational changes in their head modules, while hydrolyzing ATP and translocating DNA.
External linksProc Natl Acad Sci U S A / PubMed:35349345 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 9.0 Å
Structure data

13783

7q2x

EMDB-13783, PDB-7q2x:
Cryo-EM structure of clamped S.cerevisiae condensin-DNA complex (Form I)
Method: EM (single particle) / Resolution: 3.0 Å

13784

7q2y

EMDB-13784, PDB-7q2y:
Cryo-EM structure of clamped S.cerevisiae condensin-DNA complex (form II)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-13785: Cryo-EM map of clamped S.cerevisiae condensin-DNA complex (pentamer dataset)
Method: EM (single particle) / Resolution: 3.7 Å

13786

7q2z

EMDB-13786, PDB-7q2z:
Cryo-EM structure of S.cerevisiae condensin Ycg1-Brn1-DNA complex
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-13787: Cryo-EM map of clamped S.cerevisiae condensin complex on circular DNA
Method: EM (single particle) / Resolution: 8.75 Å

EMDB-13788: Cryo-EM map of S.cerevisiae condensin Ycg1-Brn1 complex on circular DNA
Method: EM (single particle) / Resolution: 9.0 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
  • synthetic construct (others)
KeywordsCELL CYCLE / condensin / SMC / Ycg1 / heat repeat / HAWK

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