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Structure paper

TitleStructural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases.
Journal, issue, pagesNucleic Acids Res., Vol. 49, Page 12577-12590, Year 2021
Publish dateSep 16, 2021 (structure data deposition date)
AuthorsMolina, R. / Jensen, A.L.G. / Marchena-Hurtado, J. / Lopez-Mendez, B. / Stella, S. / Montoya, G.
External linksNucleic Acids Res. / PubMed:34850143
MethodsX-ray diffraction
Resolution2.04 - 2.85 Å
Structure data

PDB-7pq2:
Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in its apo form
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-7pq3:
Crystal Structure of the Ring Nuclease 0811 from Sulfolobus islandicus (Sis0811) in complex with its post-catalytic reaction product
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-7pq6:
Crystal Structure of the Ring Nuclease 0811 mutant-S12A from Sulfolobus islandicus (Sis0811)
Method: X-RAY DIFFRACTION / Resolution: 2.67 Å

PDB-7pqa:
Crystal Structure of the Ring Nuclease 0811 mutant-S12G/K169G from Sulfolobus islandicus (Sis0811)
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-LQJ:
3'-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine

Source
  • sulfolobus islandicus rey15a (acidophilic)
KeywordsANTIVIRAL PROTEIN / RING NUCLEASE / CRISPR-ASSOCIATED PROTEIN / HELIX-TURN-HELIX / VIRAL RESISTANCE / CARF NUCLEOTIDE-BINDING DOMAIN / CRISPR Ring Nuclease

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