Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.
Journal, issue, pages	Nat Commun, Vol. 12, Issue 1, Page 3672, Year 2021
Publish date	Jun 16, 2021
Authors	Hauke S Hillen / Elena Lavdovskaia / Franziska Nadler / Elisa Hanitsch / Andreas Linden / Katherine E Bohnsack / Henning Urlaub / Ricarda Richter-Dennerlein /
PubMed Abstract	Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by ...Ribosome biogenesis requires auxiliary factors to promote folding and assembly of ribosomal proteins and RNA. Particularly, maturation of the peptidyl transferase center (PTC) is mediated by conserved GTPases, but the molecular basis is poorly understood. Here, we define the mechanism of GTPase-driven maturation of the human mitochondrial large ribosomal subunit (mtLSU) using endogenous complex purification, in vitro reconstitution and cryo-EM. Structures of transient native mtLSU assembly intermediates that accumulate in GTPBP6-deficient cells reveal how the biogenesis factors GTPBP5, MTERF4 and NSUN4 facilitate PTC folding. Addition of recombinant GTPBP6 reconstitutes late mtLSU biogenesis in vitro and shows that GTPBP6 triggers a molecular switch and progression to a near-mature PTC state. Additionally, cryo-EM analysis of GTPBP6-treated mature mitochondrial ribosomes reveals the structural basis for the dual-role of GTPBP6 in ribosome biogenesis and recycling. Together, these results provide a framework for understanding step-wise PTC folding as a critical conserved quality control checkpoint.
External links	Nat Commun / PubMed:34135319 / PubMed Central
Methods	EM (single particle)
Resolution	2.2 - 2.9 Å
Structure data	12865 7of0 EMDB-12865, PDB-7of0: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset1). Method: EM (single particle) / Resolution: 2.2 Å 12867 7of2 EMDB-12867, PDB-7of2: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with GTPBP6. Method: EM (single particle) / Resolution: 2.7 Å 12868 7of3 EMDB-12868, PDB-7of3: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 (dataset2). Method: EM (single particle) / Resolution: 2.7 Å 12869 7of4 EMDB-12869, PDB-7of4: Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 1). Method: EM (single particle) / Resolution: 2.7 Å 12870 7of5 EMDB-12870, PDB-7of5: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset2). Method: EM (single particle) / Resolution: 2.9 Å 12871 7of6 EMDB-12871, PDB-7of6: Structure of mature human mitochondrial ribosome large subunit in complex with GTPBP6 (PTC conformation 2). Method: EM (single particle) / Resolution: 2.6 Å 12872 7of7 EMDB-12872, PDB-7of7: Structure of a human mitochondrial ribosome large subunit assembly intermediate in complex with MTERF4-NSUN4 and GTPBP5 (dataset1). Method: EM (single particle) / Resolution: 2.5 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM
Source	homo sapiens (human)
Keywords	RIBOSOME / Mitochondria / Biogenesis / GTPase / NSUN4 / MTERF4