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TitleCryoEM analysis of small plant biocatalysts at sub-2 Å resolution.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 78, Issue Pt 1, Page 113-123, Year 2022
Publish dateJan 1, 2022
AuthorsNicole Dimos / Carl P O Helmer / Andrea M Chánique / Markus C Wahl / Robert Kourist / Tarek Hilal / Bernhard Loll /
PubMed AbstractEnzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse ...Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of ∼120 kDa at or below 2 Å resolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.
External linksActa Crystallogr D Struct Biol / PubMed:34981767 / PubMed Central
MethodsEM (single particle)
Resolution1.88 - 2.04 Å
Structure data

12739

7o6p

EMDB-12739, PDB-7o6p:
Structure of the borneol dehydrogenase 2 of Salvia officinalis
Method: EM (single particle) / Resolution: 2.04 Å

12740

7o6q

EMDB-12740, PDB-7o6q:
Structure of the borneol dehydrogenase 1 of salvia rosmarinus
Method: EM (single particle) / Resolution: 1.88 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • salvia officinalis (garden sage)
  • salvia rosmarinus (rosemary)
KeywordsOXIDOREDUCTASE / TERPENOID / ALCOHOL / BORNEOL / ROSSMANN-LIKE FOLD

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