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Title | Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes. |
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Journal, issue, pages | Cell Rep, Vol. 31, Issue 1, Page 107473, Year 2020 |
Publish date | Apr 7, 2020 |
Authors | Varun Bhaskar / Alexandra Graff-Meyer / Andreas D Schenk / Simone Cavadini / Ottilie von Loeffelholz / S Kundhavai Natchiar / Caroline G Artus-Revel / Hans-Rudolf Hotz / Gabriel Bretones / Bruno P Klaholz / Jeffrey A Chao / |
PubMed Abstract | Ribosomes undergo multiple conformational transitions during translation elongation. Here, we report the high-resolution cryoelectron microscopy (cryo-EM) structure of the human 80S ribosome in the ...Ribosomes undergo multiple conformational transitions during translation elongation. Here, we report the high-resolution cryoelectron microscopy (cryo-EM) structure of the human 80S ribosome in the post-decoding pre-translocation state (classical-PRE) at 3.3-Å resolution along with the rotated (hybrid-PRE) and the post-translocation states (POST). The classical-PRE state ribosome structure reveals a previously unobserved interaction between the C-terminal region of the conserved ribosomal protein uS19 and the A- and P-site tRNAs and the mRNA in the decoding site. In addition to changes in the inter-subunit bridges, analysis of different ribosomal conformations reveals the dynamic nature of this domain and suggests a role in tRNA accommodation and translocation during elongation. Furthermore, we show that disease-associated mutations in uS19 result in increased frameshifting. Together, this structure-function analysis provides mechanistic insights into the role of the uS19 C-terminal tail in the context of mammalian ribosomes. |
External links | Cell Rep / PubMed:32268098 |
Methods | EM (single particle) |
Resolution | 3.0 - 3.5 Å |
Structure data | EMDB-10668, PDB-6y0g: EMDB-10674: Cryo-EM map of human ribosome in POST state EMDB-10690: Cryo-EM map of human ribosome in hybrid-PRE state |
Chemicals | ChemComp-MG: ChemComp-3HE: ChemComp-ZN: ChemComp-HOH: |
Source |
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Keywords | RIBOSOME / Classical-PRE state / Cycloheximide / POST state / hybrid-PRE state |