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-Structure paper
Title | Structure of ribosomes from an aminoglycoside-resistant clinical isolate. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 116, Issue 44, Page 22275-22281, Year 2019 |
Publish date | Oct 29, 2019 |
Authors | Yehuda Halfon / Alicia Jimenez-Fernandez / Ruggero La Rosa / Rocio Espinosa Portero / Helle Krogh Johansen / Donna Matzov / Zohar Eyal / Anat Bashan / Ella Zimmerman / Matthew Belousoff / Søren Molin / Ada Yonath / |
PubMed Abstract | Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a ...Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a major target for clinically useful antibiotics. We report here the cryo-electron microscopy structures of the ribosome of a pathogenic aminoglycoside (AG)-resistant strain, as well as of a nonresistance strain isolated from a cystic fibrosis patient. The structural studies disclosed defective ribosome complex formation due to a conformational change of rRNA helix H69, an essential intersubunit bridge, and a secondary binding site of the AGs. In addition, a stable conformation of nucleotides A1486 and A1487, pointing into helix h44, is created compared to a non-AG-bound ribosome. We suggest that altering the conformations of ribosomal protein uL6 and rRNA helix H69, which interact with initiation-factor IF2, interferes with proper protein synthesis initiation. |
External links | Proc Natl Acad Sci U S A / PubMed:31611393 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.82 - 3.6 Å |
Structure data | EMDB-10280, PDB-6spb: EMDB-10281, PDB-6spc: EMDB-10282, PDB-6spd: EMDB-10283, PDB-6spe: EMDB-10284, PDB-6spf: EMDB-10285, PDB-6spg: |
Chemicals | ChemComp-MG: |
Source |
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Keywords | RIBOSOME / Pseudomonas aeruginosa / mutation |