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Structure paper

TitleCRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4Cleavage with ApA>p Formation Terminating RNase Activity.
Journal, issue, pagesMol. Cell, Vol. 75, Page 944-956.e6, Year 2019
Publish dateMar 7, 2019 (structure data deposition date)
AuthorsJia, N. / Jones, R. / Yang, G. / Ouerfelli, O. / Patel, D.J.
External linksMol. Cell / PubMed:31326273
MethodsX-ray diffraction
Resolution1.96 - 2.8 Å
Structure data

PDB-6o6s:
Crystal structure of Apo Csm6
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-6o6t:
Crystal structure of Csm6 H132A mutant
Method: X-RAY DIFFRACTION / Resolution: 2.31 Å

PDB-6o6v:
Crystal structure of Csm6 in complex with cA4 by soaking cA4 into Csm6
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-6o6x:
Crystal structure of Csm6 W14A/E337A mutant in complex with cA4 by cocrystallization
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-6o6y:
Crystal structure of Csm6 in complex with cyclic-tetraadenylates (cA4) by cocrystallization of Csm6 and cA4
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

PDB-6o6z:
Crystal structure of Csm6 H381A in complex with cA4 by cocrystallization of cA4 and Csm6
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6o70:
Crystal structure of Csm6 H132A mutant in complex with cA4 by cocrystallization of cA4 and Csm6 H132A mutant
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6o71:
Crystal structure of Csm6 in complex with cdA4 by soaking cdA4 into Csm6
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-6ov0:
Crystal structure of Csm6 in complex with A4>p by soaking A4>p into Csm6
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-ACK:
2',3'- cyclic AMP

ChemComp-LQJ:
3'-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine

Source
  • thermococcus onnurineus (archaea)
  • thermococcus onnurineus (strain na1) (archaea)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Type III-A CRISPR-Cas system; Csm6 / immune system/rna / Type III-A CRISPR-Cas system; Csm6 in complex with cA4 / immune system-dna complex / immune system-rna complex / Type III-A CRISPR-Cas system; Csm6 W14A-E337A mutant in complex with cA4 / Type III-A CRISPR-Cas system; Csm6 in complex with cA4 by cocrystallization of Csm6 and cA4 / Type III-A CRISPR-Cas system; Csm6 H132A mutant in complex with cA4 / immune system/dna / Type III-A CRISPR-Cas system; Csm6 in complex with cdA4

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