Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Influenza hemagglutinin membrane anchor.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 115, Issue 40, Page 10112-10117, Year 2018
Publish date	Oct 2, 2018
Authors	Donald J Benton / Andrea Nans / Lesley J Calder / Jack Turner / Ursula Neu / Yi Pu Lin / Esther Ketelaars / Nicole L Kallewaard / Davide Corti / Antonio Lanzavecchia / Steven J Gamblin / Peter B Rosenthal / John J Skehel /
PubMed Abstract	Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the ...Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the "membrane anchor" portion of the molecule. Since this region has a role in membrane fusion, we have determined its structure by analyzing the intact, full-length molecule in a detergent micelle, using cryo-EM. We have also compared the structures of full-length HA-detergent micelles with full-length HA-Fab complex detergent micelles, to describe an infectivity-neutralizing monoclonal Fab that binds near the ectodomain membrane anchor junction. We determine a high-resolution HA structure which compares favorably in detail with the structure of the ectodomain seen by X-ray crystallography; we detect, clearly, all five carbohydrate side chains of HA; and we find that the ectodomain is joined to the membrane anchor by flexible, eight-residue-long, linkers. The linkers extend into the detergent micelle to join a central triple-helical structure that is a major component of the membrane anchor.
External links	Proc Natl Acad Sci U S A / PubMed:30224494 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	1.87 - 4.2 Å
Structure data	0234 6hjn EMDB-0234, PDB-6hjn: Structure of Influenza Hemagglutinin ectodomain (A/duck/Alberta/35/76) Method: EM (single particle) / Resolution: 3.3 Å 0235 6hjp EMDB-0235, PDB-6hjp: Structure of Influenza Hemagglutinin ectodomain (A/duck/Alberta/35/76) in complex with FISW84 Fab Fragment Method: EM (single particle) / Resolution: 3.3 Å 0236 6hjq EMDB-0236, PDB-6hjq: Structure of Full-length Influenza Hemagglutinin (A/duck/Alberta/35/76) in complex with FISW84 Fab Fragment Method: EM (single particle) / Resolution: 4.1 Å 0237 6hjr EMDB-0237, PDB-6hjr: Structure of full-length Influenza Hemagglutinin with tilted transmembrane (A/duck/Alberta/35/76[H1N1]) Method: EM (single particle) / Resolution: 4.2 Å PDB-6hkg: Structure of FISW84 Fab Fragment Method: X-RAY DIFFRACTION / Resolution: 1.87 Å
Chemicals	ChemComp-UPL: UNKNOWN BRANCHED FRAGMENT OF PHOSPHOLIPID ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-SO4: SULFATE ION ChemComp-HOH: WATER
Source	influenza a virus (strain a/duck/alberta/35/1976 h1n1) homo sapiens (human)
Keywords	VIRAL PROTEIN / Influenza virus / Hemagglutinin / Membrane protein / Membrane fusion / IMMUNE SYSTEM