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Structure paper

TitleUnderstanding the indirect DNA read-out specificity of I-CreI Meganuclease.
Journal, issue, pagesSci Rep, Vol. 8, Page 10286-10286, Year 2018
Publish dateDec 18, 2017 (structure data deposition date)
AuthorsPrieto, J. / Redondo, P. / Lopez-Mendez, B. / D'Abramo, M. / Merino, N. / Blanco, F.J. / Duchateau, P. / Montoya, G. / Molina, R.
External linksSci Rep / PubMed:29980759
MethodsX-ray diffraction
Resolution2.15 - 3.024 Å
Structure data

PDB-6fb0:
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Calcium
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-6fb1:
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Magnesium
Method: X-RAY DIFFRACTION / Resolution: 3.024 Å

PDB-6fb2:
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Manganese
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-6fb5:
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Magnesium
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6fb6:
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-6fb7:
Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with its target DNA in the presence of Manganese
Method: X-RAY DIFFRACTION / Resolution: 2.689 Å

PDB-6fb8:
Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Magnesium
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-6fb9:
Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Manganese
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-PGO:
S-1,2-PROPANEDIOL

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-MN:
Unknown entry

Source
  • chlamydomonas reinhardtii (plant)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Chlamydomonas reinhardtii

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