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Structure paper

TitleStructural basis of RNA polymerase III transcription initiation.
Journal, issue, pagesNature, Vol. 553, Issue 7688, Page 301-306, Year 2018
Publish dateJan 17, 2018
AuthorsGuillermo Abascal-Palacios / Ewan Phillip Ramsay / Fabienne Beuron / Edward Morris / Alessandro Vannini /
PubMed AbstractRNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. ...RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene transcription by Pol III requires the activity of the transcription factor TFIIIB to form a transcriptionally active Pol III preinitiation complex (PIC). Here we present electron microscopy reconstructions of Pol III PICs at 3.4-4.0 Å and a reconstruction of unbound apo-Pol III at 3.1 Å. TFIIIB fully encircles the DNA and restructures Pol III. In particular, binding of the TFIIIB subunit Bdp1 rearranges the Pol III-specific subunits C37 and C34, thereby promoting DNA opening. The unwound DNA directly contacts both sides of the Pol III cleft. Topologically, the Pol III PIC resembles the Pol II PIC, whereas the Pol I PIC is more divergent. The structures presented unravel the molecular mechanisms underlying the first steps of Pol III transcription and also the general conserved mechanisms of gene transcription initiation.
External linksNature / PubMed:29345637
MethodsEM (single particle)
Resolution3.3 - 4.0 Å
Structure data

3955

6eu0

EMDB-3955, PDB-6eu0:
RNA Polymerase III open pre-initiation complex (OC-PIC)
Method: EM (single particle) / Resolution: 4.0 Å

3956

6eu1

EMDB-3956: RNA Polymerase III - open DNA complex (OC-POL3)
PDB-6eu1: RNA Polymerase III - open DNA complex (OC-POL3).
Method: EM (single particle) / Resolution: 3.4 Å

3957

6eu2

EMDB-3957, PDB-6eu2:
Apo RNA Polymerase III - open conformation (oPOL3)
Method: EM (single particle) / Resolution: 3.4 Å

3958

6eu3

EMDB-3958, PDB-6eu3:
Apo RNA Polymerase III - closed conformation (cPOL3)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-3959:
Apo RNA Polymerase III
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae s288c (yeast)
  • Saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSCRIPTION / RNA Pol III / promoter opening

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