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Structure paper

TitleCrystal structure of the mammalian lipopolysaccharide detoxifier.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 115, Page E896-E905, Year 2018
Publish dateJun 19, 2017 (structure data deposition date)
AuthorsGorelik, A. / Illes, K. / Nagar, B.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:29343645
MethodsX-ray diffraction
Resolution1.75 - 2.8 Å
Structure data

PDB-5w78:
Human acyloxyacyl hydrolase (AOAH), proteolytically processed
Method: X-RAY DIFFRACTION / Resolution: 2.271 Å

PDB-5w7a:
Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS (low quality saposin domain)
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5w7b:
Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5w7c:
Human acyloxyacyl hydrolase (AOAH), proteolytically processed, S263A mutant, with LPS
Method: X-RAY DIFFRACTION / Resolution: 2.23 Å

PDB-5w7d:
Murine acyloxyacyl hydrolase (AOAH), S262A mutant
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-5w7e:
Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with dimyristoyl phosphatidylcholine
Method: X-RAY DIFFRACTION / Resolution: 1.83 Å

PDB-5w7f:
Murine acyloxyacyl hydrolase (AOAH), S262A mutant, with lipid A
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-MYR:
MYRISTIC ACID

ChemComp-CA:
Unknown entry

ChemComp-PLM:
PALMITIC ACID

ChemComp-DAO:
LAURIC ACID

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-PO4:
PHOSPHATE ION

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-FTT:
3-HYDROXY-TETRADECANOIC ACID

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-SO4:
SULFATE ION

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-PX8:
1,2-DISTEAROYL-SN-GLYCERO-3-PHOSPHATE

ChemComp-FAW:
(2S)-3-hydroxypropane-1,2-diyl ditetradecanoate

ChemComp-GCS:
2-amino-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
  • oryctolagus cuniculus (rabbit)
  • mus musculus (house mouse)
KeywordsHYDROLASE / lipopolysaccharide / LPS / GDSL esterase / saposin

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