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Title structures of an intact type VI secretion system revealed by electron cryotomography.
Journal, issue, pagesEMBO Rep, Vol. 18, Issue 7, Page 1090-1099, Year 2017
Publish dateMay 9, 2017
AuthorsYi-Wei Chang / Lee A Rettberg / Davi R Ortega / Grant J Jensen /
PubMed AbstractThe type VI secretion system (T6SS) is a versatile molecular weapon used by many bacteria against eukaryotic hosts or prokaryotic competitors. It consists of a cytoplasmic bacteriophage tail-like ...The type VI secretion system (T6SS) is a versatile molecular weapon used by many bacteria against eukaryotic hosts or prokaryotic competitors. It consists of a cytoplasmic bacteriophage tail-like structure anchored in the bacterial cell envelope via a cytoplasmic baseplate and a periplasmic membrane complex. Rapid contraction of the sheath in the bacteriophage tail-like structure propels an inner tube/spike complex through the target cell envelope to deliver effectors. While structures of purified contracted sheath and purified membrane complex have been solved, because sheaths contract upon cell lysis and purification, no structure is available for the extended sheath. Structural information about the baseplate is also lacking. Here, we use electron cryotomography to directly visualize intact T6SS structures inside cells. Using sub-tomogram averaging, we resolve the structure of the extended sheath and membrane-associated components including the baseplate. Moreover, we identify novel extracellular bacteriophage tail fiber-like antennae. These results provide new structural insights into how the extended sheath prevents premature disassembly and how this sophisticated machine may recognize targets.
External linksEMBO Rep / PubMed:28487352 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution24.0 - 40.0 Å
Structure data

EMDB-8600:
Sub-tomogram average of the type VI secretion system membrane-associated region in Myxococcus xanthus
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

8601

5urw

EMDB-8601: Sub-tomogram average of the extended type VI secretion system sheath in Myxococcus xanthus
PDB-5urw: Structure of the extended type VI secretion system sheath in Myxococcus xanthus
Method: EM (subtomogram averaging) / Resolution: 24.0 Å

8602

5urx

EMDB-8602: Sub-tomogram average of the contracted type VI secretion system sheath in Myxococcus xanthus
PDB-5urx: Structure of the contracted type VI secretion system sheath in Myxococcus xanthus
Method: EM (subtomogram averaging) / Resolution: 28.0 Å

Source
  • Myxococcus xanthus DK 1622 (bacteria)
  • myxococcus xanthus (strain dk 1622) (bacteria)
  • myxococcus xanthus (bacteria)
KeywordsPROTEIN TRANSPORT / T6SS / type IV secretion / protein machine / secretion system / sheath / contractile

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