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TitleStructure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma.
Journal, issue, pagesNat Commun, Vol. 8, Page 14937, Year 2017
Publish dateApr 3, 2017
AuthorsKenneth Verstraete / Frank Peelman / Harald Braun / Juan Lopez / Dries Van Rompaey / Ann Dansercoer / Isabel Vandenberghe / Kris Pauwels / Jan Tavernier / Bart N Lambrecht / Hamida Hammad / Hans De Winter / Rudi Beyaert / Guy Lippens / Savvas N Savvides /
PubMed AbstractThe pro-inflammatory cytokine thymic stromal lymphopoietin (TSLP) is pivotal to the pathophysiology of widespread allergic diseases mediated by type 2 helper T cell (Th2) responses, including asthma ...The pro-inflammatory cytokine thymic stromal lymphopoietin (TSLP) is pivotal to the pathophysiology of widespread allergic diseases mediated by type 2 helper T cell (Th2) responses, including asthma and atopic dermatitis. The emergence of human TSLP as a clinical target against asthma calls for maximally harnessing its therapeutic potential via structural and mechanistic considerations. Here we employ an integrative experimental approach focusing on productive and antagonized TSLP complexes and free cytokine. We reveal how cognate receptor TSLPR allosterically activates TSLP to potentiate the recruitment of the shared interleukin 7 receptor α-chain (IL-7Rα) by leveraging the flexibility, conformational heterogeneity and electrostatics of the cytokine. We further show that the monoclonal antibody Tezepelumab partly exploits these principles to neutralize TSLP activity. Finally, we introduce a fusion protein comprising a tandem of the TSLPR and IL-7Rα extracellular domains, which harnesses the mechanistic intricacies of the TSLP-driven receptor complex to manifest high antagonistic potency.
External linksNat Commun / PubMed:28368013 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2.298 - 2.56 Å
Structure data

SASDB99:
Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex
Method: SAXS/SANS

PDB-5j11:
Structure of human TSLP in complex with TSLPR and IL-7Ralpha
Method: X-RAY DIFFRACTION / Resolution: 2.56 Å

PDB-5j13:
Structural basis for TSLP antagonism by the therapeutic antibody Tezepelumab (MEDI9929 / AMG-157)
Method: X-RAY DIFFRACTION / Resolution: 2.298 Å

Chemicals

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / cytokine inflammation TSLP signaling complex / IMMUNE SYSTEM / cytokine inflammation TSLP antibody

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