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Structure paper

TitleEnhanced alph-Zearalenol Hydrolyzing Activity of a Mycoestrogen-Detoxifying Lactonase by Structure-Based Engineering
Journal, issue, pagesAcs Catalysis, Vol. 6, Page 7657-7663, Year 2016
Publish dateFeb 25, 2016 (structure data deposition date)
AuthorsXu, Z.X. / Liu, W.D. / Chen, C.C. / Li, Q. / Huang, J.W. / Ko, T.P. / Liu, G. / Liu, W. / Peng, W. / Cheng, Y.S. ...Xu, Z.X. / Liu, W.D. / Chen, C.C. / Li, Q. / Huang, J.W. / Ko, T.P. / Liu, G. / Liu, W. / Peng, W. / Cheng, Y.S. / Chen, Y. / Jin, J. / Li, H. / Zheng, Y.Y. / Guo, R.T.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution2.39 - 2.8 Å
Structure data

PDB-5ie4:
Crystal structure of a lactonase mutant in complex with substrate a
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-5ie5:
Crystal structure of a lactonase double mutant in complex with substrate a
Method: X-RAY DIFFRACTION / Resolution: 2.39 Å

PDB-5ie6:
Crystal structure of a lactonase mutant in complex with substrate b
Method: X-RAY DIFFRACTION / Resolution: 2.67 Å

PDB-5ie7:
Crystal structure of a lactonase double mutant in complex with substrate b
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-36J:
(3S,7R,11E)-7,14,16-trihydroxy-3-methyl-3,4,5,6,7,8,9,10-octahydro-1H-2-benzoxacyclotetradecin-1-one

ChemComp-HOH:
WATER

ChemComp-ZHB:
(3S,7S,11E)-7,14,16-trihydroxy-3-methyl-3,4,5,6,7,8,9,10-octahydro-1H-2-benzoxacyclotetradecin-1-one

Source
  • clonostachys rosea (fungus)
KeywordsHYDROLASE / ALPHA/BETA-HYDROLASE / LACTONASE / ZEARALENONE

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