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Structure paper

TitleCrystallographic snapshots of sulfur insertion by lipoyl synthase.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 113, Page 9446-9450, Year 2016
Publish dateNov 23, 2015 (structure data deposition date)
AuthorsMcLaughlin, M.I. / Lanz, N.D. / Goldman, P.J. / Lee, K.H. / Booker, S.J. / Drennan, C.L.
External linksProc. Natl. Acad. Sci. USA / PubMed:27506792
MethodsX-ray diffraction
Resolution1.64 - 2.28 Å
Structure data

PDB-5exi:
Crystal structure of M. tuberculosis lipoyl synthase at 2.28 A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.28 Å

PDB-5exj:
Crystal structure of M. tuberculosis lipoyl synthase at 1.64 A resolution
Method: X-RAY DIFFRACTION / Resolution: 1.64 Å

PDB-5exk:
Crystal structure of M. tuberculosis lipoyl synthase with 6-thiooctanoyl peptide intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-DTV:
(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL

ChemComp-HOH:
WATER

ChemComp-F3S:
FE3-S4 CLUSTER

ChemComp-5AD:
5'-DEOXYADENOSINE

ChemComp-MET:
METHIONINE

ChemComp-IMD:
IMIDAZOLE

ChemComp-CL:
Unknown entry

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

Source
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
  • mycobacterium tuberculosis (bacteria)
KeywordsTRANSFERASE / auxiliary iron-sulfur cluster / AdoMet radical / radical SAM / sulfur insertion

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