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-Structure paper
Title | Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. |
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Journal, issue, pages | Acta Crystallogr D Biol Crystallogr, Vol. 70, Issue Pt 3, Page 676-684, Year 2014 |
Publish date | Feb 15, 2014 |
Authors | Piotr H Malecki / Constantinos E Vorgias / Maxim V Petoukhov / Dmitri I Svergun / Wojciech Rypniewski / |
PubMed Abstract | The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, ...The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact. |
External links | Acta Crystallogr D Biol Crystallogr / PubMed:24598737 |
Methods | SAS (X-ray synchrotron) / X-ray diffraction |
Resolution | 1.481 - 1.639 Å |
Structure data | SASDAP4: PDB-4mb3: PDB-4mb4: PDB-4mb5: |
Chemicals | ChemComp-NA: ChemComp-GLY: ChemComp-MPD: ChemComp-IMD: ChemComp-CL: ChemComp-HOH: ChemComp-GOL: ChemComp-SO4: ChemComp-PEG: |
Source |
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Keywords | HYDROLASE / TIM-barrel / alpha/beta-barrel Ig-like / Immunoglobulin like domain / ChBD / Chitin binding domain / Nag4 / Chitinase / hydrolaze / low activity mutant / Nag5 / ligand binding |