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Structure paper

TitleBacterial tubulin TubZ-Bt transitions between a two-stranded intermediate and a four-stranded filament upon GTP hydrolysis.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 111, Issue 9, Page 3407-3412, Year 2014
Publish dateMar 4, 2014
AuthorsElizabeth A Montabana / David A Agard /
PubMed AbstractCytoskeletal filaments form diverse superstructures that are highly adapted for specific functions. The recently discovered TubZ subfamily of tubulins is involved in type III plasmid partitioning ...Cytoskeletal filaments form diverse superstructures that are highly adapted for specific functions. The recently discovered TubZ subfamily of tubulins is involved in type III plasmid partitioning systems, facilitating faithful segregation of low copy-number plasmids during bacterial cell division. One such protein, TubZ-Bt, is found on the large pBtoxis plasmid in Bacillus thuringiensis, and interacts via its extended C terminus with a DNA adaptor protein TubR. Here, we use cryo-electron microscopy to determine the structure of TubZ-Bt filaments and light scattering to explore their mechanism of polymerization. Surprisingly, we find that the helical filament architecture is remarkably sensitive to nucleotide state, changing from two-stranded to four-stranded depending on the ability of TubZ-Bt to hydrolyze GTP. We present pseudoatomic models of both the two- and four-protofilament forms based on cryo-electron microscopy reconstructions (10.8 Å and 6.9 Å, respectively) of filaments formed under different nucleotide states. These data lead to a model in which the two-stranded filament is a necessary intermediate along the pathway to formation of the four-stranded filament. Such nucleotide-directed structural polymorphism is to our knowledge an unprecedented mechanism for the formation of polar filaments.
External linksProc Natl Acad Sci U S A / PubMed:24550513 / PubMed Central
MethodsEM (helical sym.)
Resolution6.8 - 10.8 Å
Structure data

5762

3j4s

EMDB-5762: Electron cryo-microscopy of four-stranded TubZ from B. thuringiensis
PDB-3j4s: Helical Model of TubZ-Bt four-stranded filament
Method: EM (helical sym.) / Resolution: 6.9 Å

5763

3j4t

EMDB-5763: Electron cryo-microscopy of two-stranded TubZ filament from B. thuringiensis
PDB-3j4t: Helical model of TubZ-Bt two-stranded filament
Method: EM (helical sym.) / Resolution: 10.8 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • bacillus thuringiensis (bacteria)
KeywordsSTRUCTURAL PROTEIN / FtsZ-like / Tubulin-like / GTPase / TubZ / plasmid segregation

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