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TitleStructures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNA.
Journal, issue, pagesSci Rep, Vol. 8, Issue 1, Page 13587, Year 2018
Publish dateSep 11, 2018
AuthorsSatabdi Mishra / Tofayel Ahmed / Anu Tyagi / Jian Shi / Shashi Bhushan /
PubMed AbstractRibosomes are the dynamic protein synthesis machineries of the cell. They may exist in different functional states in the cell. Therefore, it is essential to have structural information on these ...Ribosomes are the dynamic protein synthesis machineries of the cell. They may exist in different functional states in the cell. Therefore, it is essential to have structural information on these different functional states of ribosomes to understand their mechanism of action. Here, we present single particle cryo-EM reconstructions of the Mycobacterium smegmatis 70S ribosomes in the hibernating state (with HPF), trans-translating state (with tmRNA), and the P/P state (with P-tRNA) resolved to 4.1, 12.5, and 3.4 Å, respectively. A comparison of the P/P state with the hibernating state provides possible functional insights about the Mycobacteria-specific helix H54a rRNA segment. Interestingly, densities for all the four OB domains of bS1 protein is visible in the hibernating 70S ribosome displaying the molecular details of bS1-70S interactions. Our structural data shows a Mycobacteria-specific H54a-bS1 interaction which seems to prevent subunit dissociation and degradation during hibernation without the formation of 100S dimer. This indicates a new role of bS1 protein in 70S protection during hibernation in Mycobacteria in addition to its conserved function during translation initiation.
External linksSci Rep / PubMed:30206241 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 12.5 Å
Structure data

6920

5zeb

EMDB-6920, PDB-5zeb:
M. Smegmatis P/P state 70S ribosome structure
Method: EM (single particle) / Resolution: 3.4 Å

6921

5zep

EMDB-6921, PDB-5zep:
M. smegmatis hibernating state 70S ribosome structure
Method: EM (single particle) / Resolution: 3.4 Å

6922

5zet

EMDB-6922, PDB-5zet:
M. smegmatis P/P state 50S ribosomal subunit
Method: EM (single particle) / Resolution: 3.2 Å

6923

5zeu

EMDB-6923, PDB-5zeu:
M. smegmatis P/P state 30S ribosomal subunit
Method: EM (single particle) / Resolution: 3.7 Å

6925

5zey

EMDB-6925, PDB-5zey:
M. smegmatis Trans-translation state 70S ribosome
Method: EM (single particle) / Resolution: 12.5 Å

Source
  • mycobacterium smegmatis str. mc2 155 (bacteria)
  • escherichia coli (E. coli)
  • mycobacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria)
KeywordsRIBOSOME / translating-state / complex / hibernating-state / RNA BINDING PROTEIN/RNA / trans-translating state / RNA BINDING PROTEIN-RNA complex

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