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TitleLight-induced remodeling of phytochrome B enables signal transduction by phytochrome-interacting factor.
Journal, issue, pagesCell, Vol. 187, Issue 22, Page 6235-6250.e19, Year 2024
Publish dateOct 31, 2024
AuthorsZhengdong Wang / Wenfeng Wang / Didi Zhao / Yanping Song / Xiaoli Lin / Meng Shen / Cheng Chi / Bin Xu / Jun Zhao / Xing Wang Deng / Jizong Wang /
PubMed AbstractPhytochrome B (phyB) and phytochrome-interacting factors (PIFs) constitute a well-established signaling module critical for plants adapting to ambient light. However, mechanisms underlying phyB ...Phytochrome B (phyB) and phytochrome-interacting factors (PIFs) constitute a well-established signaling module critical for plants adapting to ambient light. However, mechanisms underlying phyB photoactivation and PIF binding for signal transduction remain elusive. Here, we report the cryo-electron microscopy (cryo-EM) structures of the photoactivated phyB or the constitutively active phyB mutant in complex with PIF6, revealing a similar trimer. The light-induced configuration switch of the chromophore drives a conformational transition of the nearby tongue signature within the phytochrome-specific (PHY) domain of phyB. The resulting α-helical PHY tongue further disrupts the head-to-tail dimer of phyB in the dark-adapted state. These structural remodelings of phyB facilitate the induced-fit recognition of PIF6, consequently stabilizing the N-terminal extension domain and a head-to-head dimer of activated phyB. Interestingly, the phyB dimer exhibits slight asymmetry, resulting in the binding of only one PIF6 molecule. Overall, our findings solve a key question with respect to how light-induced remodeling of phyB enables PIF signaling in phytochrome research.
External linksCell / PubMed:39317197
MethodsEM (single particle)
Resolution3.1 - 3.19 Å
Structure data

39108

8yb4

EMDB-39108, PDB-8yb4:
Pfr conformer of Arabidopsis thaliana phytochrome B in complex with phytochrome-interacting factor 6
Method: EM (single particle) / Resolution: 3.1 Å

60916

9iuz

EMDB-60916, PDB-9iuz:
Constitutively active mutant(Y276H) of Arabidopsis phytochrome B(phyB) in complex with phytochrome-interacting factor 6(PIF6)
Method: EM (single particle) / Resolution: 3.19 Å

Chemicals

ChemComp-O6E:
3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid

Source
  • arabidopsis thaliana (thale cress)
KeywordsGENE REGULATION / phytochrome / phytochrome interaction factor / signal complex / SIGNALING PROTEIN / Phytochrome-interacting factor

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