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TitleIn situ structural analysis of the Yersinia enterocolitica injectisome.
Journal, issue, pagesElife, Vol. 2, Page e00792, Year 2013
Publish dateJul 30, 2013
AuthorsMikhail Kudryashev / Marco Stenta / Stefan Schmelz / Marlise Amstutz / Ulrich Wiesand / Daniel Castaño-Díez / Matteo T Degiacomi / Stefan Münnich / Christopher Ke Bleck / Julia Kowal / Andreas Diepold / Dirk W Heinz / Matteo Dal Peraro / Guy R Cornelis / Henning Stahlberg /
PubMed AbstractInjectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the ...Injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the first three-dimensional structure of Yersinia enterocolitica and Shigella flexneri injectisomes in situ and the first structural analysis of the Yersinia injectisome. Unexpectedly, basal bodies of injectisomes inside the bacterial cells showed length variations of 20%. The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had similar dimensions and were significantly longer than the isolated structures of related injectisomes. The crystal structure of the inner membrane injectisome component YscD appeared elongated compared to a homologous protein, and molecular dynamics simulations documented its elongation elasticity. The ring-shaped secretin YscC at the outer membrane was stretched by 30-40% in situ, compared to its isolated liposome-embedded conformation. We suggest that elasticity is critical for some two-membrane spanning protein complexes to cope with variations in the intermembrane distance. DOI:http://dx.doi.org/10.7554/eLife.00792.001.
External linksElife / PubMed:23908767 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution1.4 - 40.0 Å
Structure data

EMDB-5694:
Type III secretion system (Injectisome) of Yersinia enterocolitica in situ
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

PDB-4alz:
The Yersinia T3SS basal body component YscD reveals a different structural periplasmatic domain organization to known homologue PrgH
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • yersinia enterocolitica (bacteria)
KeywordsMEMBRANE PROTEIN / T3SS / TYPE 3 SECRETION / EFFECTOR TRANSPORT

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