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Structure paper

TitleStructural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 3814, Year 2019
Publish dateAug 23, 2019
AuthorsSarah V Faull / Andy M C Lau / Chloe Martens / Zainab Ahdash / Kjetil Hansen / Hugo Yebenes / Carla Schmidt / Fabienne Beuron / Nora B Cronin / Edward P Morris / Argyris Politis /
PubMed AbstractCullin-Ring E3 Ligases (CRLs) regulate a multitude of cellular pathways through specific substrate receptors. The COP9 signalosome (CSN) deactivates CRLs by removing NEDD8 from activated Cullins. ...Cullin-Ring E3 Ligases (CRLs) regulate a multitude of cellular pathways through specific substrate receptors. The COP9 signalosome (CSN) deactivates CRLs by removing NEDD8 from activated Cullins. Here we present structures of the neddylated and deneddylated CSN-CRL2 complexes by combining single-particle cryo-electron microscopy (cryo-EM) with chemical cross-linking mass spectrometry (XL-MS). These structures suggest a conserved mechanism of CSN activation, consisting of conformational clamping of the CRL2 substrate by CSN2/CSN4, release of the catalytic CSN5/CSN6 heterodimer and finally activation of the CSN5 deneddylation machinery. Using hydrogen-deuterium exchange (HDX)-MS we show that CRL2 activates CSN5/CSN6 in a neddylation-independent manner. The presence of NEDD8 is required to activate the CSN5 active site. Overall, by synergising cryo-EM with MS, we identify sensory regions of the CSN that mediate its stepwise activation and provide a framework for understanding the regulatory mechanism of other Cullin family members.
External linksNat Commun / PubMed:31444342 / PubMed Central
MethodsEM (single particle)
Resolution5.9 - 8.8 Å
Structure data

4736

6r6h

EMDB-4736, PDB-6r6h:
Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
Method: EM (single particle) / Resolution: 8.4 Å

4739

6r7f

EMDB-4739, PDB-6r7f:
Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
Method: EM (single particle) / Resolution: 8.2 Å

4741

6r7h

EMDB-4741, PDB-6r7h:
Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
Method: EM (single particle) / Resolution: 8.8 Å

4742

6r7i

EMDB-4742, PDB-6r7i:
Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
Method: EM (single particle) / Resolution: 5.9 Å

4744

6r7n

EMDB-4744, PDB-6r7n:
Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
Method: EM (single particle) / Resolution: 6.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsLIGASE / Cullin-Ring E3 Ligase COP9 Signalosome Neddylation / Cullin-Ring E3 Ligases (CRLs) COP9 signalosome (CSN) deneddylation / Cullin-Ring E3 Ligases (CRLs) COP9 signalosome (CSN) deneddylation VHL tumour suppressor substrate receptor

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