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Title | Square condenser apertures for square cameras in low-dose transmission electron microscopy. |
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Journal, issue, pages | Nat Methods, Vol. 21, Issue 4, Page 566-568, Year 2024 |
Publish date | Mar 8, 2024 |
Authors | Hamish G Brown / Dan Smith / Benjamin C Wardle / Eric Hanssen / |
PubMed Abstract | In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam- ...In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam-sensitive samples. We present condenser aperture plates with square and rectangular shapes that improve the efficiency of area usage by 70% and enhance montage imaging for beam-sensitive specimens. We demonstrate the compatibility of these condenser aperture plates with high-resolution cryogenic TEM by reconstructing a 1.8-Å map of equine apo-ferritin. |
External links | Nat Methods / PubMed:38459386 |
Methods | EM (single particle) |
Resolution | 1.76 - 2.8 Å |
Structure data | EMDB-41933: CryoEM map of horse spleen apoferritin determined as a reference for benchmarking square and rectangular apertures for cryo-EM EMDB-41936: CryoEM map of horse spleen apoferritin determined as a reference for benchmarking square and rectangular apertures for cryo-EM (Falcon IV round beam) EMDB-41937: CryoEM map of horse spleen apoferritin determined for benchmarking square and rectangular apertures for cryo-EM (Falcon IV square beam) EMDB-41938: CryoEM map of horse spleen apoferritin determined for benchmarking square and rectangular apertures for cryo-EM (Gatan K3 rectangular beam) EMDB-42882: Amylin 1 receptor bound to salmon calcitonin (Amy1R:sCT) reconstructed from cryo-EM datasets recorded using a rectangular aperture |
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