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TitleImplications of a multiscale structure of the yeast nuclear pore complex.
Journal, issue, pagesMol Cell, Vol. 83, Issue 18, Page 3283-33302.e5, Year 2023
Publish dateSep 21, 2023
AuthorsChristopher W Akey / Ignacia Echeverria / Christna Ouch / Ilona Nudelman / Yi Shi / Junjie Wang / Brian T Chait / Andrej Sali / Javier Fernandez-Martinez / Michael P Rout /
PubMed AbstractNuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from ...Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution.
External linksMol Cell / PubMed:37738963 / PubMed Central
MethodsEM (single particle)
Resolution6.6 - 31.7 Å
Structure data

EMDB-41114: Nuclear double outer ring of the yeast NPC
Method: EM (single particle) / Resolution: 23.0 Å

EMDB-41116: Lumenal ring of the isolated yeast NPC
Method: EM (single particle) / Resolution: 14.7 Å

41117

8t9l

EMDB-41117, PDB-8t9l:
Pom34-Pom152 membrane attachment site yeast NPC
Method: EM (single particle) / Resolution: 7.0 Å

EMDB-41119: Cytoplasmic outer ring of the yeast NPC
Method: EM (single particle) / Resolution: 22.0 Å

EMDB-41120: Central transporter/plug yeast NPC
Method: EM (single particle) / Resolution: 28.0 Å

EMDB-41121: Double outer ring connectors of the yeast NPC
Method: EM (single particle) / Resolution: 29.5 Å

EMDB-41122: Connectors in cytoplasmic outer ring of yeast NPC
Method: EM (single particle) / Resolution: 31.7 Å

EMDB-41123: Composite multiscale structure of the yeast NPC
Method: EM (single particle) / Resolution: 28.0 Å

41285

8tie

EMDB-41285, PDB-8tie:
Double nuclear outer ring of Nup84-complexes from the yeast NPC
Method: EM (single particle) / Resolution: 8.1 Å

41300

8tj5

EMDB-41300, PDB-8tj5:
Inner spoke ring of the yeast NPC
Method: EM (single particle) / Resolution: 6.6 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSPORT PROTEIN / nuclear pore complex / nucleocytoplasmic transport / nucleoporin / membrane protein / translocase

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