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-Structure paper
Title | CEP104/FAP256 and associated cap complex maintain stability of the ciliary tip. |
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Journal, issue, pages | J Cell Biol, Vol. 222, Issue 11, Year 2023 |
Publish date | Nov 6, 2023 |
Authors | Thibault Legal / Mireya Parra / Maxwell Tong / Corbin S Black / Ewa Joachimiak / Melissa Valente-Paterno / Karl Lechtreck / Jacek Gaertig / Khanh Huy Bui / |
PubMed Abstract | Cilia are essential organelles that protrude from the cell body. Cilia are made of a microtubule-based structure called the axoneme. In most types of cilia, the ciliary tip is distinct from the rest ...Cilia are essential organelles that protrude from the cell body. Cilia are made of a microtubule-based structure called the axoneme. In most types of cilia, the ciliary tip is distinct from the rest of the cilium. Here, we used cryo-electron tomography and subtomogram averaging to obtain the structure of the ciliary tip of the ciliate Tetrahymena thermophila. We show that the microtubules at the tip are highly crosslinked with each other and stabilized by luminal proteins, plugs, and cap proteins at the plus ends. In the tip region, the central pair lacks typical projections and twists significantly. By analyzing cells lacking a ciliary tip-enriched protein CEP104/FAP256 by cryo-electron tomography and proteomics, we discovered candidates for the central pair cap complex and explained the potential functions of CEP104/FAP256. These data provide new insights into the function of the ciliary tip and the mechanisms of ciliary assembly and length regulation. |
External links | J Cell Biol / PubMed:37756660 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (tomography) |
Resolution | 8.8 - 130.0 Å |
Structure data | EMDB-40891: Main central pair structure EMDB-40892: Ciliary cap complex from Tetrahymena thermophila EMDB-40894: Singlet A-tubule from Tetrahymena thermophila EMDB-40896: Tip central pair consensus map from Tetrahymena thermophila cilia EMDB-40897: C1 refinement of the tip central pair subtomogram average EMDB-40898: C1 MAPS refinement of ciliary tip central pair subtomogram average EMDB-40899: C2 refinement of ciliary tip central pair subtomogram average EMDB-40900: C2 MAPs refinement of ciliary tip central pair subtomogram average EMDB-40906: Ciliary tip central pair composite map from Tetrahymena thermophila EMDB-40909: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (consensus map) EMDB-40910: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (C1 refinement) EMDB-40911: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (C2 refinement) EMDB-40912: Ciliary tip central pair map of FAP256-KO Tetrahymena thermophila (composite map) EMDB-40913: Ciliary tip tomogram of Tetrahymena thermophila |
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