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TitleStructure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.
Journal, issue, pagesCell, Vol. 166, Issue 6, Page 1436-1444.e10, Year 2016
Publish dateSep 8, 2016
AuthorsTiago R D Costa / Aravindan Ilangovan / Marta Ukleja / Adam Redzej / Joanne M Santini / Terry K Smith / Edward H Egelman / Gabriel Waksman /
PubMed AbstractConjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among ...Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 Å resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection.
External linksCell / PubMed:27610568 / PubMed Central
MethodsEM (helical sym.)
Resolution3.6 - 5.0 Å
Structure data

4042

5leg

EMDB-4042: Cryo-EM structure of the bacterial sex F pilus (pED208)
PDB-5leg: Structure of the bacterial sex F pilus (pED208)
Method: EM (helical sym.) / Resolution: 3.6 Å

4044

5ler

EMDB-4044: Cryo-EM structure of the bacterial sex F pilus (13.2 Angstrom rise)
PDB-5ler: Structure of the bacterial sex F pilus (13.2 Angstrom rise)
Method: EM (helical sym.) / Resolution: 5.0 Å

4046

5lfb

EMDB-4046: Cryo-EM structure of the bacterial sex F pilus (12.5 Angstrom rise)
PDB-5lfb: Structure of the bacterial sex F pilus (12.5 Angstrom rise)
Method: EM (helical sym.) / Resolution: 5.0 Å

Chemicals

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-6V6:
[(2~{S})-3-[[(2~{R})-2,3-bis(oxidanyl)propoxy]-oxidanyl-phosphoryl]oxy-2-hexadec-9-enoyloxy-propyl] hexadecanoate

Source
  • escherichia coli (E. coli)
  • salmonella enterica subsp. enterica serovar typhi (bacteria)
KeywordsPROTEIN FIBRIL / F-pilus Conjugation Type IV secretion system Phospholipid / Cell adhesion

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