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TitleStructural basis of promoter recognition by Staphylococcus aureus RNA polymerase.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 4850, Year 2024
Publish dateJun 6, 2024
AuthorsLinggang Yuan / Qingyang Liu / Liqiao Xu / Bing Wu / Yu Feng /
PubMed AbstractBacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ controls housekeeping functions, S. aureus σ regulates virulence, biofilm formation, ...Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While Staphylococcus aureus σ controls housekeeping functions, S. aureus σ regulates virulence, biofilm formation, persistence, cell internalization, membrane transport, and antimicrobial resistance. Besides the sequence difference, the spacers between the -35 element and -10 element of σ regulated promoters are shorter than those of σ regulated promoters. Therefore, how σ recognizes and initiates transcription from target promoters can not be inferred from that of the well studied σ. Here, we report the cryo-EM structures of S. aureus RNAP-promoter open complexes comprising σ and σ, respectively. Structural analyses, in combination with biochemical experiments, reveal the structural basis for the promoter specificity of S. aureus transcription. Although the -10 element of σ regulated promoters is recognized by domain σ as single-stranded DNA, the -10 element of σ regulated promoters is co-recognized by domains σ and σ as double-stranded DNA, accounting for the short spacers of σ regulated promoters. S. aureus RNAP is a validated target of antibiotics, and our structures pave the way for rational drug design targeting S. aureus RNAP.
External linksNat Commun / PubMed:38844782 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 3.7 Å
Structure data

38087

8x6f

EMDB-38087, PDB-8x6f:
Cryo-EM structure of Staphylococcus aureus sigA-dependent RNAP-promoter open complex
Method: EM (single particle) / Resolution: 3.7 Å

38088

8x6g

EMDB-38088, PDB-8x6g:
Cryo-EM structure of Staphylococcus aureus sigB-dependent RNAP-promoter open complex
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • staphylococcus aureus (bacteria)
KeywordsTRANSCRIPTION/DNA / RNA polymerase / transcription / Staphylococcus aureus / sigA / TRANSCRIPTION-DNA complex / sigB

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