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-Structure paper
Title | Structures of the mumps virus polymerase complex via cryo-electron microscopy. |
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Journal, issue, pages | Nat Commun, Vol. 15, Issue 1, Page 4189, Year 2024 |
Publish date | May 17, 2024 |
Authors | Tianhao Li / Mingdong Liu / Zhanxi Gu / Xin Su / Yunhui Liu / Jinzhong Lin / Yu Zhang / Qing-Tao Shen / |
PubMed Abstract | The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), ...The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), while structures corresponding to these activities remain obscure. Here, we resolved two L-P complex conformations from the mumps virus (MuV), a typical member of nsNSVs, via cryogenic-electron microscopy. One conformation presents all five domains of L forming a continuous RNA tunnel to the methyltransferase domain (MTase), preferably as a transcription state. The other conformation has the appendage averaged out, which is inaccessible to MTase. In both conformations, parallel P tetramers are revealed around MuV L, which, together with structures of other nsNSVs, demonstrates the diverse origins of the L-binding X domain of P. Our study links varying structures of nsNSV polymerase complexes with genome replication and transcription and points to a sliding model for polymerase complexes to advance along the RNA templates. |
External links | Nat Commun / PubMed:38760379 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.93 - 3.63 Å |
Structure data | EMDB-35864: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer (composite map) EMDB-37957: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer EMDB-37958: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for CD-MTase-CTD) EMDB-37959: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for RdRp-PRNTase) EMDB-37960: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for tetrameric phosphoproteins) EMDB-37961: Cryo-EM map for Mumps Virus L Protein (State 2) Bound by Phosphoprotein Tetramer EMDB-37962: Cryo-EM map for Mumps Virus L protein (state2) Bound by Phosphoprotein Tetramer (Focused for tetrameric phosphoprotein) EMDB-37964: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer (composite map) |
Chemicals | ChemComp-ZN: |
Source |
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Keywords | VIRAL PROTEIN / Mumps virus polymerase complex / RNA-dependent RNA synthesis / Large protein / phosphoprotein. |