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TitleStructures of the mumps virus polymerase complex via cryo-electron microscopy.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 4189, Year 2024
Publish dateMay 17, 2024
AuthorsTianhao Li / Mingdong Liu / Zhanxi Gu / Xin Su / Yunhui Liu / Jinzhong Lin / Yu Zhang / Qing-Tao Shen /
PubMed AbstractThe viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), ...The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), while structures corresponding to these activities remain obscure. Here, we resolved two L-P complex conformations from the mumps virus (MuV), a typical member of nsNSVs, via cryogenic-electron microscopy. One conformation presents all five domains of L forming a continuous RNA tunnel to the methyltransferase domain (MTase), preferably as a transcription state. The other conformation has the appendage averaged out, which is inaccessible to MTase. In both conformations, parallel P tetramers are revealed around MuV L, which, together with structures of other nsNSVs, demonstrates the diverse origins of the L-binding X domain of P. Our study links varying structures of nsNSV polymerase complexes with genome replication and transcription and points to a sliding model for polymerase complexes to advance along the RNA templates.
External linksNat Commun / PubMed:38760379 / PubMed Central
MethodsEM (single particle)
Resolution2.93 - 3.63 Å
Structure data

EMDB-35864: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer (composite map)
PDB-8izl: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-37957: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer
Method: EM (single particle) / Resolution: 3.02 Å

EMDB-37958: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for CD-MTase-CTD)
PDB-8yxl: Structure of C-terminal domain of L protein from Mumps virus
Method: EM (single particle) / Resolution: 3.13 Å

EMDB-37959: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for RdRp-PRNTase)
PDB-8yxm: Structure of N-terminal domain of L protein bound with Phosphoprotein from Mumps Virus
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-37960: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for tetrameric phosphoproteins)
PDB-8yxo: Structure of Phosphoprotein tetramer from mumps virus
Method: EM (single particle) / Resolution: 3.49 Å

EMDB-37961: Cryo-EM map for Mumps Virus L Protein (State 2) Bound by Phosphoprotein Tetramer
PDB-8yxp: Structure of mumps virus L protein (state2)
Method: EM (single particle) / Resolution: 3.01 Å

EMDB-37962: Cryo-EM map for Mumps Virus L protein (state2) Bound by Phosphoprotein Tetramer (Focused for tetrameric phosphoprotein)
PDB-8yxr: Structure of Phosphoprotein Tetramer from mumps virus
Method: EM (single particle) / Resolution: 3.63 Å

EMDB-37964: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer (composite map)
PDB-8x01: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer
Method: EM (single particle) / Resolution: 3.01 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • mumps orthorubulavirus
KeywordsVIRAL PROTEIN / Mumps virus polymerase complex / RNA-dependent RNA synthesis / Large protein / phosphoprotein.

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