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TitleInhibition and transport mechanisms of the ABC transporter hMRP5.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 4811, Year 2024
Publish dateJun 6, 2024
AuthorsYing Huang / Chenyang Xue / Ruiqian Bu / Cang Wu / Jiachen Li / Jinqiu Zhang / Jinyu Chen / Zhaoying Shi / Yonglong Chen / Yong Wang / Zhongmin Liu /
PubMed AbstractHuman multidrug resistance protein 5 (hMRP5) effluxes anticancer and antivirus drugs, driving multidrug resistance. To uncover the mechanism of hMRP5, we determine six distinct cryo-EM structures, ...Human multidrug resistance protein 5 (hMRP5) effluxes anticancer and antivirus drugs, driving multidrug resistance. To uncover the mechanism of hMRP5, we determine six distinct cryo-EM structures, revealing an autoinhibitory N-terminal peptide that must dissociate to permit subsequent substrate recruitment. Guided by these molecular insights, we design an inhibitory peptide that could block substrate entry into the transport pathway. We also identify a regulatory motif, comprising a positively charged cluster and hydrophobic patches, within the first nucleotide-binding domain that modulates hMRP5 localization by engaging with membranes. By integrating our structural, biochemical, computational, and cell biological findings, we propose a model for hMRP5 conformational cycling and localization. Overall, this work provides mechanistic understanding of hMRP5 function, while informing future selective hMRP5 inhibitor development. More broadly, this study advances our understanding of the structural dynamics and inhibition of ABC transporters.
External linksNat Commun / PubMed:38844452 / PubMed Central
MethodsEM (single particle)
Resolution2.93 - 4.06 Å
Structure data

37105

8kci

EMDB-37105, PDB-8kci:
ATP-bound hMRP5 outward-open
Method: EM (single particle) / Resolution: 3.94 Å

37554

8wi0

EMDB-37554, PDB-8wi0:
wt-hMRP5 inward-open
Method: EM (single particle) / Resolution: 2.93 Å

37555

8wi2

EMDB-37555, PDB-8wi2:
RD-hMRP5-inward open
Method: EM (single particle) / Resolution: 4.06 Å

37556

8wi3

EMDB-37556, PDB-8wi3:
ND-hMRP5-inward open
Method: EM (single particle) / Resolution: 3.38 Å

37557

8wi4

EMDB-37557, PDB-8wi4:
m6-hMRP5 inward open
Method: EM (single particle) / Resolution: 3.2 Å

37558

8wi5

EMDB-37558, PDB-8wi5:
M5PI-bound hMRP5
Method: EM (single particle) / Resolution: 3.46 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / ATP-bound hMRP5 outward-open / wt-hMRP5 inward-open / RD-hMRP5-inward open / ND-hMRP5 / m6-hMRP5 inward open / M5PI-bound hMRP5

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