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Title | Structural basis for an early stage of the photosystem II repair cycle in Chlamydomonas reinhardtii. |
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Journal, issue, pages | Nat Commun, Vol. 15, Issue 1, Page 5211, Year 2024 |
Publish date | Jun 18, 2024 |
Authors | Anjie Li / Tingting You / Xiaojie Pang / Yidi Wang / Lijin Tian / Xiaobo Li / Zhenfeng Liu / |
PubMed Abstract | Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs ...Photosystem II (PSII) catalyzes water oxidation and plastoquinone reduction by utilizing light energy. It is highly susceptible to photodamage under high-light conditions and the damaged PSII needs to be restored through a process known as the PSII repair cycle. The detailed molecular mechanism underlying the PSII repair process remains mostly elusive. Here, we report biochemical and structural features of a PSII-repair intermediate complex, likely arrested at an early stage of the PSII repair process in the green alga Chlamydomonas reinhardtii. The complex contains three protein factors associated with a damaged PSII core, namely Thylakoid Enriched Factor 14 (TEF14), Photosystem II Repair Factor 1 (PRF1), and Photosystem II Repair Factor 2 (PRF2). TEF14, PRF1 and PRF2 may facilitate the release of the manganese-stabilizing protein PsbO, disassembly of peripheral light-harvesting complexes from PSII and blockage of the Q site, respectively. Moreover, an α-tocopherol quinone molecule is located adjacent to the heme group of cytochrome b, potentially fulfilling a photoprotective role by preventing the generation of reactive oxygen species. |
External links | Nat Commun / PubMed:38890314 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.6 - 3.2 Å |
Structure data | EMDB-37133, PDB-8kde: EMDB-37265: Overall cryo-EM map of an intermediate-state complex during the process of photosystem II repair EMDB-37288: A focused cryo-EM map of an intermediate-state complex during the process of photosystem II repair (Part1) EMDB-37289: A focused cryo-EM map of an intermediate-state complex during the process of photosystem II repair (Part 2) EMDB-60026, PDB-8zee: |
Chemicals | ChemComp-CLA: ChemComp-BCR: ChemComp-LMG: ChemComp-LHG: ChemComp-PL9: ChemComp-PHO: ChemComp-HEM: ChemComp-VTQ: ChemComp-DGD: ChemComp-SQD: ChemComp-BCT: ChemComp-FE2: |
Source |
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Keywords | PHOTOSYNTHESIS / monomeric Photosystem II / PSII repair |