+Search query
-Structure paper
Title | Metallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy. |
---|---|
Journal, issue, pages | Commun Biol, Vol. 7, Issue 1, Page 65, Year 2024 |
Publish date | Jan 9, 2024 |
Authors | Yixin Xu / Yuqi Qin / Lang Wang / Yingyi Zhang / Yufeng Wang / Shangyu Dang / |
PubMed Abstract | Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck in the current ...Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck in the current single particle cryo-EM pipeline is the preparation of good quality frozen cryo-EM grids, which is mostly a trial-and-error process. Among many issues, preferred particle orientation and sample damage by air-water interface (AWI) are common practical problems. Here we report a method of applying metallo-supramolecular branched polymer (MSBP) in the cryo-sample preparation for high-resolution single-particle cryo-EM. Our data shows that MSBP keeps a majority of particles away from air-water interface and mitigates preferred orientation as verified by the analyses of apoferritin, hemagglutinin) trimer and various sample proteins. The use of MSBP is a simple method to improve particle distribution for high-resolution structure determination in single-particle cryo-EM. |
External links | Commun Biol / PubMed:38195919 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.16 - 3.19 Å |
Structure data | EMDB-36313: Cryo-EM structure of apoferritin with MSBP EMDB-36314: Cryo-EM structure of hemagglutinin with MSBP EMDB-36315: Cryo-EM structure of catalase with MSBP EMDB-36316: Cryo-EM structure of beta-Galactosidase with MSBP |
Source |
|