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| Title | Cryo-EM structure of cyanophage P-SCSP1u offers insights into DNA gating and evolution of T7-like viruses. |
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| Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 6438, Year 2023 |
| Publish date | Oct 13, 2023 |
 Authors | Lanlan Cai / Hang Liu / Wen Zhang / Shiwei Xiao / Qinglu Zeng / Shangyu Dang /  |
| PubMed Abstract | Cyanophages, together with their host cyanobacteria, play important roles in marine biogeochemical cycles and control of marine food webs. The recently identified MPP-C (Marine Picocyanobacteria ...Cyanophages, together with their host cyanobacteria, play important roles in marine biogeochemical cycles and control of marine food webs. The recently identified MPP-C (Marine Picocyanobacteria Podovirus clade C) cyanophages, belonging to the T7-like podoviruses, contain the smallest genomes among cyanopodoviruses and exhibit distinct infection kinetics. However, understanding of the MPP-C cyanophage infection process is hindered by the lack of high-resolution structural information. Here, we report the cryo-EM structure of the cyanophage P-SCSP1u, a representative member of the MPP-C phages, in its native form at near-atomic resolution, which reveals the assembly mechanism of the capsid and molecular interaction of the portal-tail complex. Structural comparison of the capsid proteins of P-SCSP1u and other podoviruses with known structures provides insights into the evolution of T7-like viruses. Furthermore, our study provides the near-atomic resolution structure of portal-tail complex for T7-like viruses. On the basis of previously reported structures of phage T7, we identify an additional valve and gate to explain the DNA gating mechanism for the T7-like viruses. |
 External links | Nat Commun / PubMed:37833330 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.23 - 3.81 Å |
| Structure data | EMDB-35174, PDB-8i4l: EMDB-35175, PDB-8i4m: |
| Source |
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 Keywords | VIRUS / Whole virus / Capsid / cyanophage / T7-like virus |
prochlorococcus phage p-scsp1u (virus)