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TitleFunctional selectivity of insulin receptor revealed by aptamer-trapped receptor structures.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 6500, Year 2022
Publish dateOct 30, 2022
AuthorsJunhong Kim / Na-Oh Yunn / Mangeun Park / Jihan Kim / Seongeun Park / Yoojoong Kim / Jeongeun Noh / Sung Ho Ryu / Yunje Cho /
PubMed AbstractActivation of insulin receptor (IR) initiates a cascade of conformational changes and autophosphorylation events. Herein, we determined three structures of IR trapped by aptamers using cryo-electron ...Activation of insulin receptor (IR) initiates a cascade of conformational changes and autophosphorylation events. Herein, we determined three structures of IR trapped by aptamers using cryo-electron microscopy. The A62 agonist aptamer selectively activates metabolic signaling. In the absence of insulin, the two A62 aptamer agonists of IR adopt an insulin-accessible arrowhead conformation by mimicking site-1/site-2' insulin coordination. Insulin binding at one site triggers conformational changes in one protomer, but this movement is blocked in the other protomer by A62 at the opposite site. A62 binding captures two unique conformations of IR with a similar stalk arrangement, which underlie Tyr1150 mono-phosphorylation (m-pY1150) and selective activation for metabolic signaling. The A43 aptamer, a positive allosteric modulator, binds at the opposite side of the insulin-binding module, and stabilizes the single insulin-bound IR structure that brings two FnIII-3 regions into closer proximity for full activation. Our results suggest that spatial proximity of the two FnIII-3 ends is important for m-pY1150, but multi-phosphorylation of IR requires additional conformational rearrangement of intracellular domains mediated by coordination between extracellular and transmembrane domains.
External linksNat Commun / PubMed:36310231 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 4.27 Å
Structure data

34018

7yq3

EMDB-34018, PDB-7yq3:
human insulin receptor bound with A43 DNA aptamer and insulin
Method: EM (single particle) / Resolution: 3.6 Å

34019

7yq4

EMDB-34019, PDB-7yq4:
human insulin receptor bound with A62 DNA aptamer and insulin - locally refined
Method: EM (single particle) / Resolution: 3.95 Å

34020

7yq5

EMDB-34020, PDB-7yq5:
human insulin receptor bound with A62 DNA aptamer and insulin
Method: EM (single particle) / Resolution: 4.27 Å

34021

7yq6

EMDB-34021, PDB-7yq6:
human insulin receptor bound with A62 DNA aptamer
Method: EM (single particle) / Resolution: 4.18 Å

34281

8guy

EMDB-34281, PDB-8guy:
human insulin receptor bound with two insulin molecules
Method: EM (single particle) / Resolution: 4.18 Å

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsSTRUCTURAL PROTEIN / receptor-ligand complex / receptor-ligand complex_B_local / receptor-ligand complex_B_overall / receptor-ligand complex_C

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