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Structure paper

TitleTranscription initiation complex structures elucidate DNA opening.
Journal, issue, pagesNature, Vol. 533, Issue 7603, Page 353-358, Year 2016
Publish dateMay 19, 2016
AuthorsC Plaschka / M Hantsche / C Dienemann / C Burzinski / J Plitzko / P Cramer /
PubMed AbstractTranscription of eukaryotic protein-coding genes begins with assembly of the RNA polymerase (Pol) II initiation complex and promoter DNA opening. Here we report cryo-electron microscopy (cryo-EM) ...Transcription of eukaryotic protein-coding genes begins with assembly of the RNA polymerase (Pol) II initiation complex and promoter DNA opening. Here we report cryo-electron microscopy (cryo-EM) structures of yeast initiation complexes containing closed and open DNA at resolutions of 8.8 Å and 3.6 Å, respectively. DNA is positioned and retained over the Pol II cleft by a network of interactions between the TATA-box-binding protein TBP and transcription factors TFIIA, TFIIB, TFIIE, and TFIIF. DNA opening occurs around the tip of the Pol II clamp and the TFIIE 'extended winged helix' domain, and can occur in the absence of TFIIH. Loading of the DNA template strand into the active centre may be facilitated by movements of obstructing protein elements triggered by allosteric binding of the TFIIE 'E-ribbon' domain. The results suggest a unified model for transcription initiation with a key event, the trapping of open promoter DNA by extended protein-protein and protein-DNA contacts.
External linksNature / PubMed:27193681
MethodsEM (single particle) / X-ray diffraction
Resolution3.52 - 8.8 Å
Structure data

EMDB-3375:
Transcription initiation complex structures elucidate DNA opening (OC1)
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-3376:
Transcription initiation complex structures elucidate promoter DNA opening (OC2)
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-3377:
Transcription initiation complex structures elucidate DNA opening (OC2-focused)
Method: EM (single particle) / Resolution: 4.71 Å

3378

5fyw

EMDB-3378: Transcription initiation complex structures elucidate DNA opening (OC3)
PDB-5fyw: Transcription initiation complex structures elucidate DNA opening (OC)
Method: EM (single particle) / Resolution: 4.35 Å

EMDB-3379:
Transcription initiation complex structures elucidate DNA opening (OC3-focused)
Method: EM (single particle) / Resolution: 7.5 Å

EMDB-3380:
Transcription initiation complex structures elucidate DNA opening (OC4)
Method: EM (single particle) / Resolution: 3.89 Å

EMDB-3381:
Transcription initiation complex structures elucidate DNA opening (OC4-focused)
Method: EM (single particle) / Resolution: 4.09 Å

EMDB-3382:
Transcription initiation complex structures elucidate DNA opening (OC5)
Method: EM (single particle) / Resolution: 6.1 Å

3383

5fz5

EMDB-3383, PDB-5fz5:
Transcription initiation complex structures elucidate DNA opening (CC)
Method: EM (single particle) / Resolution: 8.8 Å

PDB-5ip7:
Structure of RNA Polymerase II-Tfg1 peptide complex
Method: X-RAY DIFFRACTION / Resolution: 3.52 Å

PDB-5ip9:
Structure of RNA Polymerase II-TFIIF complex
Method: X-RAY DIFFRACTION / Resolution: 3.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • unidentified (others)
  • synthetic construct (others)
  • synthetic (others)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsTRANSCRIPTION / GENE EXPRESSION / TRANSCRIPTION INITIATION

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