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TitleCryo-EM structures of human Cx36/GJD2 neuronal gap junction channel.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 1347, Year 2023
Publish dateMar 11, 2023
AuthorsSeu-Na Lee / Hwa-Jin Cho / Hyeongseop Jeong / Bumhan Ryu / Hyuk-Joon Lee / Minsoo Kim / Jejoong Yoo / Jae-Sung Woo / Hyung Ho Lee /
PubMed AbstractConnexin 36 (Cx36) is responsible for signal transmission in electrical synapses by forming interneuronal gap junctions. Despite the critical role of Cx36 in normal brain function, the molecular ...Connexin 36 (Cx36) is responsible for signal transmission in electrical synapses by forming interneuronal gap junctions. Despite the critical role of Cx36 in normal brain function, the molecular architecture of the Cx36 gap junction channel (GJC) is unknown. Here, we determine cryo-electron microscopy structures of Cx36 GJC at 2.2-3.6 Å resolutions, revealing a dynamic equilibrium between its closed and open states. In the closed state, channel pores are obstructed by lipids, while N-terminal helices (NTHs) are excluded from the pore. In the open state with pore-lining NTHs, the pore is more acidic than those in Cx26 and Cx46/50 GJCs, explaining its strong cation selectivity. The conformational change during channel opening also includes the α-to-π-helix transition of the first transmembrane helix, which weakens the protomer-protomer interaction. Our structural analyses provide high resolution information on the conformational flexibility of Cx36 GJC and suggest a potential role of lipids in the channel gating.
External linksNat Commun / PubMed:36906653 / PubMed Central
MethodsEM (single particle)
Resolution2.2 - 7.2 Å
Structure data

33254

7xki

EMDB-33254, PDB-7xki:
Human Cx36/GJD2 (N-terminal deletion BRIL-fused mutant) gap junction channel in soybean lipids (D6 symmetry)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-33255: Human Cx36/GJD2 (N-terminal deletion BRIL-fused mutant) gap junction channel in soybean lipids (C1 symmetry)
Method: EM (single particle) / Resolution: 4.5 Å

33256

7xkk

EMDB-33256, PDB-7xkk:
Human Cx36/GJD2 gap junction channel in detergents
Method: EM (single particle) / Resolution: 3.2 Å

33270

7xkt

EMDB-33270, PDB-7xkt:
Human Cx36/GJD2 (BRIL-fused mutant) gap junction channel in detergents at 2.2 Angstroms resolution
Method: EM (single particle) / Resolution: 2.2 Å

33274

7xl8

EMDB-33274, PDB-7xl8:
Human Cx36/GJD2 (N-terminal deletion mutant) gap junction channel in soybean lipids (D6 symmetry)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-33275: Human Cx36/GJD2 (N-terminal deletion mutant) gap junction channel in soybean lipids (C1 symmetry)
Method: EM (single particle) / Resolution: 3.41 Å

33315

7xnh

EMDB-33315, PDB-7xnh:
Human Cx36/GJD2 gap junction channel with pore-lining N-terminal helices in soybean lipids
Method: EM (single particle) / Resolution: 3.1 Å

33327

7xnv

EMDB-33327, PDB-7xnv:
Structurally hetero-junctional human Cx36/GJD2 gap junction channel in soybean lipids (C6 symmetry)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-33328: Structurally hetero-junctional human Cx36/GJD2 gap junction channel in soybean lipids (C1 symmetry)
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-34822: Human Cx36/GJD2 (BRIL-fused mutant) gap junction channel in soybean lipids
Method: EM (single particle) / Resolution: 3.4 Å

34856

8hkp

EMDB-34856, PDB-8hkp:
Structurally hetero-junctional human Cx36/GJD2 gap junction channel in detergents (C6 symmetry)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-34857: Structurally hetero-junctional human Cx36/GJD2 gap junction channel in detergents (C1 symmetry)
Method: EM (single particle) / Resolution: 7.2 Å

Chemicals

ChemComp-MC3:
1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-HOH:
WATER

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / connexin 36 / Gap Junction Channel / Cx36 / GJD2 / BRIL

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