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TitleStructural and mechanistic insights into fungal β-1,3-glucan synthase FKS1.
Journal, issue, pagesNature, Vol. 616, Issue 7955, Page 190-198, Year 2023
Publish dateMar 22, 2023
AuthorsXinlin Hu / Ping Yang / Changdong Chai / Jia Liu / Huanhuan Sun / Yanan Wu / Mingjie Zhang / Min Zhang / Xiaotian Liu / Hongjun Yu /
PubMed AbstractThe membrane-integrated synthase FKS is involved in the biosynthesis of β-1,3-glucan, the core component of the fungal cell wall. FKS is the target of widely prescribed antifungal drugs, including ...The membrane-integrated synthase FKS is involved in the biosynthesis of β-1,3-glucan, the core component of the fungal cell wall. FKS is the target of widely prescribed antifungal drugs, including echinocandin and ibrexafungerp. Unfortunately, the mechanism of action of FKS remains enigmatic and this has hampered development of more effective medicines targeting the enzyme. Here we present the cryo-electron microscopy structures of Saccharomyces cerevisiae FKS1 and the echinocandin-resistant mutant FKS1(S643P). These structures reveal the active site of the enzyme at the membrane-cytoplasm interface and a glucan translocation path spanning the membrane bilayer. Multiple bound lipids and notable membrane distortions are observed in the FKS1 structures, suggesting active FKS1-membrane interactions. Echinocandin-resistant mutations are clustered at a region near TM5-6 and TM8 of FKS1. The structure of FKS1(S643P) reveals altered lipid arrangements in this region, suggesting a drug-resistant mechanism of the mutant enzyme. The structures, the catalytic mechanism and the molecular insights into drug-resistant mutations of FKS1 revealed in this study advance the mechanistic understanding of fungal β-1,3-glucan biosynthesis and establish a foundation for developing new antifungal drugs by targeting FKS.
External linksNature / PubMed:36949198 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 3.5 Å
Structure data

33154

7xe4

EMDB-33154, PDB-7xe4:
structure of a membrane-bound glycosyltransferase
Method: EM (single particle) / Resolution: 3.4 Å

34115

7yuy

EMDB-34115, PDB-7yuy:
Structure of a mutated membrane-bound glycosyltransferase
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-DD9:
nonane

ChemComp-D10:
DECANE

ChemComp-C14:
TETRADECANE

ChemComp-HP6:
HEPTANE

ChemComp-D12:
DODECANE

ChemComp-XKP:
(11R,14S)-17-amino-14-hydroxy-8,14-dioxo-9,13,15-trioxa-14lambda~5~-phosphaheptadecan-11-yl decanoate

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • baker's yeast (brewer's yeast)
KeywordsTRANSFERASE / membrane protein / glycosyltransferase

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