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Structure paper

TitleStructures of the human cholecystokinin receptors bound to agonists and antagonists.
Journal, issue, pagesNat Chem Biol, Vol. 17, Issue 12, Page 1230-1237, Year 2021
Publish dateSep 23, 2021
AuthorsXuefeng Zhang / Chenglin He / Mu Wang / Qingtong Zhou / Dehua Yang / Ya Zhu / Wenbo Feng / Hui Zhang / Antao Dai / Xiaojing Chu / Jia Wang / Zhenlin Yang / Yi Jiang / Ulrich Sensfuss / Qiuxiang Tan / Shuo Han / Steffen Reedtz-Runge / H Eric Xu / Suwen Zhao / Ming-Wei Wang / Beili Wu / Qiang Zhao /
PubMed AbstractCholecystokinin receptors, CCKR and CCKR, are important neurointestinal peptide hormone receptors and play a vital role in food intake and appetite regulation. Here, we report three crystal ...Cholecystokinin receptors, CCKR and CCKR, are important neurointestinal peptide hormone receptors and play a vital role in food intake and appetite regulation. Here, we report three crystal structures of the human CCKR in complex with different ligands, including one peptide agonist and two small-molecule antagonists, as well as two cryo-electron microscopy structures of CCKR-gastrin in complex with G and G, respectively. These structures reveal the recognition pattern of different ligand types and the molecular basis of peptide selectivity in the cholecystokinin receptor family. By comparing receptor structures in different conformational states, a stepwise activation process of cholecystokinin receptors is proposed. Combined with pharmacological data, our results provide atomic details for differential ligand recognition and receptor activation mechanisms. These insights will facilitate the discovery of potential therapeutics targeting cholecystokinin receptors.
External linksNat Chem Biol / PubMed:34556863
MethodsEM (single particle) / X-ray diffraction
Resolution2.5 - 3.3 Å
Structure data

31493

7f8v

EMDB-31493, PDB-7f8v:
Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gi
Method: EM (single particle) / Resolution: 3.3 Å

31494

7f8w

EMDB-31494, PDB-7f8w:
Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gq
Method: EM (single particle) / Resolution: 3.1 Å

PDB-7f8u:
Crystal structure of the cholecystokinin receptor CCKAR in complex with lintitript
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-7f8x:
Crystal structure of the cholecystokinin receptor CCKAR in complex with NN9056
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-7f8y:
Crystal structure of the cholecystokinin receptor CCKAR in complex with devazepide
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-1OE:
2-[2-[[4-(2-chlorophenyl)-1,3-thiazol-2-yl]carbamoyl]indol-1-yl]ethanoic acid

ChemComp-1OZ:
N-[(3S)-1-methyl-2-oxidanylidene-5-phenyl-3H-1,4-benzodiazepin-3-yl]-1H-indole-2-carboxamide / antagonist*YM

Source
  • homo sapiens (human)
  • enterobacteria phage t4 (virus)
  • escherichia virus t4
  • synthetic construct (others)
KeywordsSTRUCTURAL PROTEIN / G protein-coulped receptor / Cholecystokinin receptor CCKAR / lintitript / Cholecystokinin receptor CCKBR / Gastrin-17 / devazepide

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