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TitleNative doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 2168, Year 2023
Publish dateApr 15, 2023
AuthorsShintaroh Kubo / Corbin S Black / Ewa Joachimiak / Shun Kai Yang / Thibault Legal / Katya Peri / Ahmad Abdelzaher Zaki Khalifa / Avrin Ghanaeian / Caitlyn L McCafferty / Melissa Valente-Paterno / Chelsea De Bellis / Phuong M Huynh / Zhe Fan / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui /
PubMed AbstractCilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine ...Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.
External linksNat Commun / PubMed:37061538 / PubMed Central
MethodsEM (subtomogram averaging) / EM (single particle)
Resolution3.7 - 22.0 Å
Structure data

EMDB-29666: 96-nm repeat unit of intact doublet microtubule from Tetrahymena thermophila CFAP77AB-KO mutant
Method: EM (subtomogram averaging) / Resolution: 22.0 Å

EMDB-29667: 96-nm repeat unit of intact doublet microtubule from Tetrahymena thermophila strain CU428
Method: EM (subtomogram averaging) / Resolution: 19.0 Å

29685

8g2z

EMDB-29685, PDB-8g2z:
48-nm doublet microtubule from Tetrahymena thermophila strain CU428
Method: EM (single particle) / Resolution: 4.1 Å

29692

8g3d

EMDB-29692, PDB-8g3d:
48-nm doublet microtubule from Tetrahymena thermophila strain K40R
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-29693: 96-nm doublet microtubule from combined Tetrahymena thermophila strains CU428 and K40R
Method: EM (single particle) / Resolution: 3.75 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • tetrahymena thermophila (eukaryote)
KeywordsSTRUCTURAL PROTEIN / Cilia / Axoneme / Doublet Microtubule / Microtubule Inner Protein

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