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-Structure paper
Title | Structure and assembly of the NOT10:11 module of the CCR4-NOT complex. |
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Journal, issue, pages | Commun Biol, Vol. 6, Issue 1, Page 739, Year 2023 |
Publish date | Jul 17, 2023 |
Authors | Yevgen Levdansky / Tobias Raisch / Justin C Deme / Filip Pekovic / Hans Elmlund / Susan M Lea / Eugene Valkov / |
PubMed Abstract | NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT ...NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly. |
External links | Commun Biol / PubMed:37460791 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.57 - 2.88 Å |
Structure data | EMDB-29551, PDB-8fy3: EMDB-29552, PDB-8fy4: |
Source |
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Keywords | RNA BINDING PROTEIN / mRNA degradation / gene expression |