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TitleRegulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement.
Journal, issue, pagesCell, Vol. 158, Issue 1, Page 121-131, Year 2014
Publish dateJul 3, 2014
AuthorsTatyana V Budkevich / Jan Giesebrecht / Elmar Behrmann / Justus Loerke / David J F Ramrath / Thorsten Mielke / Jochen Ismer / Peter W Hildebrand / Chang-Shung Tung / Knud H Nierhaus / Karissa Y Sanbonmatsu / Christian M T Spahn /
PubMed AbstractThe extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution ...The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1A⋅Val-tRNA⋅GMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection.
External linksCell / PubMed:24995983 / PubMed Central
MethodsEM (single particle)
Resolution6.9 - 8.9 Å
Structure data

EMDB-2620: POST-translocational 80S ribosomal state of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
PDB-4uje: Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-2621:
PRE-translocational (classical-1) 80S ribosomal state of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
Method: EM (single particle) / Resolution: 7.6 Å

EMDB-2622:
PRE-translocational (classical-2) 80S ribosomal state of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
Method: EM (single particle) / Resolution: 7.5 Å

EMDB-2623: Sampling intermediate of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
PDB-4cxg: Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
Method: EM (single particle) / Resolution: 8.7 Å

EMDB-2624: Recognition intermediate of Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
PDB-4cxh: Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
Method: EM (single particle) / Resolution: 8.9 Å

Chemicals

ChemComp-PHE:
PHENYLALANINE

Source
  • oryctolagus cuniculus (rabbit)
  • escherichia coli (E. coli)
  • bos taurus (cattle)
KeywordsTRANSLATION / MAMMALIAN 80S RIBOSOME / ELONGATION CYCLE / TRNA SELECTION / EUKARYOTIC TERNARY COMPLEX / ELONGATION FACTOR EEF1A / RIBOSOME / POST-TRANSLOCATIONAL STATE

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