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Structure paper

TitleStructural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide
Journal, issue, pagesNat Commun, Vol. 13, Page 2747, Year 2022
Publish dateSep 2, 2021 (structure data deposition date)
AuthorsZhao Y / Roy K / Vidossich P / Cancedda L / De Vivo M / Forbush B / Cao E
External linksNat Commun / Search PubMed
MethodsEM (single particle)
Resolution2.9 - 3.6 Å
Structure data

24807

7s1x

EMDB-24807, PDB-7s1x:
Cryo-EM structure of human NKCC1 K289NA492EL671C bound with bumetanide
Method: EM (single particle) / Resolution: 2.9 Å

24812

7s1y

EMDB-24812, PDB-7s1y:
Cryo-EM structure of human NKCC1 K289NA492E bound with bumetanide
Method: EM (single particle) / Resolution: 3.6 Å

24813

7s1z

EMDB-24813, PDB-7s1z:
Cryo-EM structure of Human NKCC1 K289NA492EL671C
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-26588: Human NKCC1 K289NA492E Bound with Furosemide
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-82U:
3-(butylamino)-4-phenoxy-5-sulfamoylbenzoic acid / medication*YM

ChemComp-K:
Unknown entry

ChemComp-CL:
Unknown entry

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / cation-chloride cotransporter / ion transport

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