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Title | Structural basis for voltage-sensor trapping of the cardiac sodium channel by a deathstalker scorpion toxin. |
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Journal, issue, pages | Nat Commun, Vol. 12, Issue 1, Page 128, Year 2021 |
Publish date | Jan 4, 2021 |
![]() | Daohua Jiang / Lige Tonggu / Tamer M Gamal El-Din / Richard Banh / Régis Pomès / Ning Zheng / William A Catterall / ![]() ![]() |
PubMed Abstract | Voltage-gated sodium (Na) channels initiate action potentials in excitable cells, and their function is altered by potent gating-modifier toxins. The α-toxin LqhIII from the deathstalker scorpion ...Voltage-gated sodium (Na) channels initiate action potentials in excitable cells, and their function is altered by potent gating-modifier toxins. The α-toxin LqhIII from the deathstalker scorpion inhibits fast inactivation of cardiac Na1.5 channels with IC = 11.4 nM. Here we reveal the structure of LqhIII bound to Na1.5 at 3.3 Å resolution by cryo-EM. LqhIII anchors on top of voltage-sensing domain IV, wedged between the S1-S2 and S3-S4 linkers, which traps the gating charges of the S4 segment in a unique intermediate-activated state stabilized by four ion-pairs. This conformational change is propagated inward to weaken binding of the fast inactivation gate and favor opening the activation gate. However, these changes do not permit Na permeation, revealing why LqhIII slows inactivation of Na channels but does not open them. Our results provide important insights into the structural basis for gating-modifier toxin binding, voltage-sensor trapping, and fast inactivation of Na channels. |
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Methods | EM (single particle) |
Resolution | 3.3 Å |
Structure data | EMDB-22621, PDB-7k18: |
Chemicals | ![]() ChemComp-NAG: ![]() ChemComp-6OU: |
Source |
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![]() | MEMBRANE PROTEIN / Ion channel / Toxin bound |