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TitlePre-termination Transcription Complex: Structure and Function.
Journal, issue, pagesMol Cell, Vol. 81, Issue 2, Page 281-292.e8, Year 2021
Publish dateJan 21, 2021
AuthorsZhitai Hao / Vitaly Epshtein / Kelly H Kim / Sergey Proshkin / Vladimir Svetlov / Venu Kamarthapu / Binod Bharati / Alexander Mironov / Thomas Walz / Evgeny Nudler /
PubMed AbstractRho is a general transcription termination factor playing essential roles in RNA polymerase (RNAP) recycling, gene regulation, and genomic stability in most bacteria. Traditional models of ...Rho is a general transcription termination factor playing essential roles in RNA polymerase (RNAP) recycling, gene regulation, and genomic stability in most bacteria. Traditional models of transcription termination postulate that hexameric Rho loads onto RNA prior to contacting RNAP and then translocates along the transcript in pursuit of the moving RNAP to pull RNA from it. Here, we report the cryoelectron microscopy (cryo-EM) structures of two termination process intermediates. Prior to interacting with RNA, Rho forms a specific "pre-termination complex" (PTC) with RNAP and elongation factors NusA and NusG, which stabilize the PTC. RNA exiting RNAP interacts with NusA before entering the central channel of Rho from the distal C-terminal side of the ring. We map the principal interactions in the PTC and demonstrate their critical role in termination. Our results support a mechanism in which the formation of a persistent PTC is a prerequisite for termination.
External linksMol Cell / PubMed:33296676 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 7.7 Å
Structure data

22114

6xas

EMDB-22114, PDB-6xas:
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex
Method: EM (single particle) / Resolution: 3.8 Å

22115

6xav

EMDB-22115, PDB-6xav:
CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex bound with NusG
Method: EM (single particle) / Resolution: 7.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (strain k12) (bacteria)
  • escherichia coli k-12 (bacteria)
KeywordsTRANSCRIPTION / Rho-dependent transcription termination

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