+Search query
-Structure paper
Title | Structural and functional characterization of the bestrophin-2 anion channel. |
---|---|
Journal, issue, pages | Nat Struct Mol Biol, Vol. 27, Issue 4, Page 382-391, Year 2020 |
Publish date | Apr 6, 2020 |
Authors | Aaron P Owji / Qingqing Zhao / Changyi Ji / Alec Kittredge / Austin Hopiavuori / Ziao Fu / Nancy Ward / Oliver B Clarke / Yin Shen / Yu Zhang / Wayne A Hendrickson / Tingting Yang / |
PubMed Abstract | The bestrophin family of calcium (Ca)-activated chloride (Cl) channels, which mediate the influx and efflux of monovalent anions in response to the levels of intracellular Ca, comprises four members ...The bestrophin family of calcium (Ca)-activated chloride (Cl) channels, which mediate the influx and efflux of monovalent anions in response to the levels of intracellular Ca, comprises four members in mammals (bestrophin 1-4). Here we report cryo-EM structures of bovine bestrophin-2 (bBest2) bound and unbound by Ca at 2.4- and 2.2-Å resolution, respectively. The bBest2 structure highlights four previously underappreciated pore-lining residues specifically conserved in Best2 but not in Best1, illustrating the differences between these paralogs. Structure-inspired electrophysiological analysis reveals that, although the channel is sensitive to Ca, it has substantial Ca-independent activity for Cl, reflecting the opening at the cytoplasmic restriction of the ion conducting pathway even when Ca is absent. Moreover, the ion selectivity of bBest2 is controlled by multiple residues, including those involved in gating. |
External links | Nat Struct Mol Biol / PubMed:32251414 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.17 - 3.03 Å |
Structure data | EMDB-21430, PDB-6vx5: EMDB-21431, PDB-6vx6: EMDB-21432, PDB-6vx7: EMDB-21433: bestrophin-2 (BEST2) calcium-unbound state 2 (EGTA only) EMDB-21434: Bestrophin-2 (BEST2) calcium-unbound state 1 (EGTA only) |
Chemicals | ChemComp-CL: ChemComp-CA: ChemComp-HOH: |
Source |
|
Keywords | MEMBRANE PROTEIN / Chloride channel |