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TitleAtomic structures of anthrax toxin protective antigen channels bound to partially unfolded lethal and edema factors.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 840, Year 2020
Publish dateFeb 11, 2020
AuthorsNathan J Hardenbrook / Shiheng Liu / Kang Zhou / Koyel Ghosal / Z Hong Zhou / Bryan A Krantz /
PubMed AbstractFollowing assembly, the anthrax protective antigen (PA) forms an oligomeric translocon that unfolds and translocates either its lethal factor (LF) or edema factor (EF) into the host cell. Here, we ...Following assembly, the anthrax protective antigen (PA) forms an oligomeric translocon that unfolds and translocates either its lethal factor (LF) or edema factor (EF) into the host cell. Here, we report the cryo-EM structures of heptameric PA channels with partially unfolded LF and EF at 4.6 and 3.1-Å resolution, respectively. The first α helix and β strand of LF and EF unfold and dock into a deep amphipathic cleft, called the α clamp, which resides at the interface of two PA monomers. The α-clamp-helix interactions exhibit structural plasticity when comparing the structures of lethal and edema toxins. EF undergoes a largescale conformational rearrangement when forming the complex with the channel. A critical loop in the PA binding interface is displaced for about 4 Å, leading to the weakening of the binding interface prior to translocation. These structures provide key insights into the molecular mechanisms of translocation-coupled protein unfolding and translocation.
External linksNat Commun / PubMed:32047164 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 4.6 Å
Structure data

20459

6psn

EMDB-20459, PDB-6psn:
Anthrax toxin protective antigen channels bound to lethal factor
Method: EM (single particle) / Resolution: 4.6 Å

20955

6uzb

EMDB-20955, PDB-6uzb:
Anthrax toxin protective antigen channels bound to edema factor
Method: EM (single particle) / Resolution: 3.2 Å

20957

6uzd

EMDB-20957, PDB-6uzd:
Anthrax toxin protective antigen channels bound to edema factor
Method: EM (single particle) / Resolution: 3.4 Å

20958

6uze

EMDB-20958, PDB-6uze:
Anthrax toxin protective antigen channels bound to edema factor
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-CA:
Unknown entry

Source
  • bacillus anthracis (anthrax bacterium)
KeywordsTRANSLOCASE / anthrax toxin / protective antigen / lethal factor / edema factor

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