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TitleKatanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.
Journal, issue, pagesDev Cell, Vol. 52, Issue 1, Page 118-131.e6, Year 2020
Publish dateJan 6, 2020
AuthorsElena A Zehr / Agnieszka Szyk / Ewa Szczesna / Antonina Roll-Mecak /
PubMed AbstractThe AAA ATPase katanin severs microtubules. It is critical in cell division, centriole biogenesis, and neuronal morphogenesis. Its mutation causes microcephaly. The microtubule templates katanin ...The AAA ATPase katanin severs microtubules. It is critical in cell division, centriole biogenesis, and neuronal morphogenesis. Its mutation causes microcephaly. The microtubule templates katanin hexamerization and activates its ATPase. The structural basis for these activities and how they lead to severing is unknown. Here, we show that β-tubulin tails are necessary and sufficient for severing. Cryoelectron microscopy (cryo-EM) structures reveal the essential tubulin tail glutamates gripped by a double spiral of electropositive loops lining the katanin central pore. Each spiral couples allosterically to the ATPase and binds alternating, successive substrate residues, with consecutive residues coordinated by adjacent protomers. This tightly couples tail binding, hexamerization, and ATPase activation. Hexamer structures in different states suggest an ATPase-driven, ratchet-like translocation of the tubulin tail through the pore. A disordered region outside the AAA core anchors katanin to the microtubule while the AAA motor exerts the forces that extract tubulin dimers and sever the microtubule.
External linksDev Cell / PubMed:31735665 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 4.2 Å
Structure data

20761

6ugd

EMDB-20761, PDB-6ugd:
Katanin hexamer in the spiral conformation in complex with substrate
Method: EM (single particle) / Resolution: 3.5 Å

20762

6uge

EMDB-20762, PDB-6uge:
Katanin hexamer in the ring conformation in complex with substrate
Method: EM (single particle) / Resolution: 3.6 Å

20763

6ugf

EMDB-20763, PDB-6ugf:
Katanin hexamer in the ring conformation with resolved protomer one in complex with substrate
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • caenorhabditis elegans (invertebrata)
  • synthetic construct (others)
KeywordsMOTOR PROTEIN / katanin / microtubule-severing / MEI-1 / microtubule cytoskeleton

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