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Structure paper

TitleThe cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 4288, Year 2019
Publish dateSep 19, 2019
AuthorsSiew Siew Pang / Charles Bayly-Jones / Mazdak Radjainia / Bradley A Spicer / Ruby H P Law / Adrian W Hodel / Edward S Parsons / Susan M Ekkel / Paul J Conroy / Georg Ramm / Hariprasad Venugopal / Phillip I Bird / Bart W Hoogenboom / Ilia Voskoboinik / Yann Gambin / Emma Sierecki / Michelle A Dunstone / James C Whisstock /
PubMed AbstractMacrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, ...Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 Å structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 Å cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated β-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets.
External linksNat Commun / PubMed:31537793 / PubMed Central
MethodsEM (single particle)
Resolution2.37 - 8.94 Å
Structure data

20616

6u23

EMDB-20616, PDB-6u23:
EM structure of MPEG-1(w.t.) soluble pre-pore
Method: EM (single particle) / Resolution: 3.49 Å

EMDB-20617:
EM map of MPEG-1 (w.t.) soluble pre-pore complex
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-20619:
EM map of MPEG-1(L425K, alpha conformation) soluble pre-pore complex
Method: EM (single particle) / Resolution: 3.01 Å

20620

6u2j

EMDB-20620, PDB-6u2j:
EM structure of MPEG-1 (L425K, alpha conformation) soluble pre-pore complex
Method: EM (single particle) / Resolution: 2.37 Å

20621

6u2k

EMDB-20621, PDB-6u2k:
EM structure of MPEG-1 (L425K, alpha conformation) soluble pre-pore complex
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-20622:
EM map of MPEG-1(w.t.) pre-pore complex bound to liposome
Method: EM (single particle) / Resolution: 8.94 Å

20623

6u2l

EMDB-20623, PDB-6u2l:
EM structure of MPEG-1 (L425K, beta conformation) soluble pre-pore complex
Method: EM (single particle) / Resolution: 2.83 Å

20627

6u2w

EMDB-20627, PDB-6u2w:
EM structure of MPEG-1(L425K) pre-pore complex bound to liposome
Method: EM (single particle) / Resolution: 3.63 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / MPEG-1/Perforin-2 / MACPF / macrophage / pore forming proteins / immunity

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