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Structure paper

TitleStructural Intermediates of the Dimeric Kinesin Stepping Cycle Revealed by Cryo-EM
Journal, issue, pagesTo Be Published
Publish dateApr 12, 2019 (structure data deposition date)
AuthorsCha HK / Debs G / Liu X / Liu D / Sindelar CV
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.23 - 7.55 Å
Structure data

20092

6ojq

EMDB-20092, PDB-6ojq:
Monomeric kinesin-1 motor domain in no-nucleotide state bound to GMPCPP-stabilized microtubule
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-20093:
Cryo-EM reconstruction of the 'x1x' binding pattern in the low ADP condition for dimeric kinesin-1 with a central bound site and flanking sites with either bound or empty occupancy
Method: EM (single particle) / Resolution: 3.23 Å

EMDB-20094:
Cryo-EM reconstruction of the '010' binding pattern in the low ADP condition for dimeric kinesin-1 with a central bound site and empty flanking sites
Method: EM (single particle) / Resolution: 3.31 Å

EMDB-20095:
Cryo-EM reconstruction of the '0110' binding pattern in the low ADP condition for dimeric kinesin-1 with two central bound sites and empty flanking sites
Method: EM (single particle) / Resolution: 3.75 Å

EMDB-20096:
Cryo-EM reconstruction of the '010' binding pattern in the ADP-AlFx condition for dimeric kinesin-1 with a central bound site and empty flanking sites
Method: EM (single particle) / Resolution: 5.63 Å

EMDB-20097:
Cryo-EM reconstruction of the 'x11x' binding pattern in the ADP-AlFx condition for dimeric kinesin-1 with two central bound sites and flanking sites with either empty or bound occupancy
Method: EM (single particle) / Resolution: 4.8 Å

EMDB-20098:
Cryo-EM reconstruction of the '0110' binding pattern in the ADP-Pi condition for dimeric kinesin-1 with two central bound sites and empty flanking sites
Method: EM (single particle) / Resolution: 7.55 Å

Chemicals

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • bos taurus (cattle)
  • homo sapiens (human)
  • homo s (cattle)
KeywordsMOTOR PROTEIN / Kinesin / Microtubule

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