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TitleDiatom pyrenoids are encased in a protein shell that enables efficient CO fixation.
Journal, issue, pagesCell, Vol. 187, Issue 21, Page 5919-5934.e19, Year 2024
Publish dateOct 17, 2024
AuthorsGinga Shimakawa / Manon Demulder / Serena Flori / Akihiro Kawamoto / Yoshinori Tsuji / Hermanus Nawaly / Atsuko Tanaka / Rei Tohda / Tadayoshi Ota / Hiroaki Matsui / Natsumi Morishima / Ryosuke Okubo / Wojciech Wietrzynski / Lorenz Lamm / Ricardo D Righetto / Clarisse Uwizeye / Benoit Gallet / Pierre-Henri Jouneau / Christoph Gerle / Genji Kurisu / Giovanni Finazzi / Benjamin D Engel / Yusuke Matsuda /
PubMed AbstractPyrenoids are subcompartments of algal chloroplasts that increase the efficiency of Rubisco-driven CO fixation. Diatoms fix up to 20% of global CO, but their pyrenoids remain poorly characterized. ...Pyrenoids are subcompartments of algal chloroplasts that increase the efficiency of Rubisco-driven CO fixation. Diatoms fix up to 20% of global CO, but their pyrenoids remain poorly characterized. Here, we used in vivo photo-crosslinking to identify pyrenoid shell (PyShell) proteins, which we localized to the pyrenoid periphery of model pennate and centric diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana. In situ cryo-electron tomography revealed that pyrenoids of both diatom species are encased in a lattice-like protein sheath. Single-particle cryo-EM yielded a 2.4-Å-resolution structure of an in vitro TpPyShell1 lattice, which showed how protein subunits interlock. T. pseudonana TpPyShell1/2 knockout mutants had no PyShell sheath, altered pyrenoid morphology, and a high-CO requiring phenotype, with reduced photosynthetic efficiency and impaired growth under standard atmospheric conditions. The structure and function of the diatom PyShell provide a molecular view of how CO is assimilated in the ocean, a critical ecosystem undergoing rapid change.
External linksCell / PubMed:39357521
MethodsEM (subtomogram averaging) / EM (tomography) / EM (helical sym.)
Resolution2.4 - 20.0 Å
Structure data

EMDB-18709: Subtomogram average of the T. pseudonana PyShell
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

EMDB-18710: Tomogram of P. tricornutum pyrenoid
Method: EM (tomography)

EMDB-18711: Tomogram of T. pseudonana pyrenoid
Method: EM (tomography)

EMDB-18712: Tomogram of PyShell mutant (M1) T. pseudonana
Method: EM (tomography)

EMDB-18713: Tomogram of PyShell mutant (M2) T. pseudonana
Method: EM (tomography)

EMDB-18841: Tomogram of T. pseudonana pyrenoid used for subtomogram avergaing
Method: EM (tomography)

37751

8wqp

EMDB-37751, PDB-8wqp:
Cryo-EM structure of T. pseudonana PyShell helical tube
Method: EM (helical sym.) / Resolution: 2.4 Å

Source
  • thalassiosira pseudonana (Diatom)
KeywordsPLANT PROTEIN / marine diatoms / photosynthesis / CO2-concentrating mechanism / pyrenoids

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